Taxonomic properties of a sourgrass isolate of maize dwarf mosaic virus in Taiwan.
A virus (SCMV-Sou) isolated from wild Paspalum conjugatum in Taiwan was designated from host range tests as maize dwarf mosaic potyvirus str. A (MDMV-A). Assayed in the maize cv. Tainan No.5, the thermal inactivation point was 50-55°C, dilution end-point 10-4 - 10-5 and longevity in vitro 1-2 d. The MW of capsid protein estimated by SDS-PAGE was 45 100 daltons. In serological tests MDMV-A was discernible from MDMV-B or other strains of sugarcane mosaic potyvirus by SDS-immunodiffusion even though there were cross-reactions among these viruses. The viruses were not clearly distinguishable from one another in immunosorbent electron microscopy but numerous filamentous particles were observed in partially purified preparations of MDMV-A. MDMV-A, MDMV-B and strains of SCMV were individually detected by DAS-ELISA, each antiserum being cross-absorbed with heterologous antigen.