Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

Micropropagation of Dendrocalamus hamiltonii Munro using single node cuttings taken from elite seedling plants.

Abstract

Single-node cuttings of precocious branchlets were cultured on MS medium as a source of explants (3-4 mm segments from newly initiated buds) for further culture. Direct shoot bud regeneration leading to multiple shoot initiation and/or callus formation was achieved on MS medium containing 1 mg/litre of each of benzyladenine and 2,4-D. Addition of 0.5 mg gibberellic acid (GA3)/litre to the medium encouraged shoot differentiation in callus cultures. Some albino and variegated shoots were obtained, although most were green. Rapid shoot multiplication was achieved in half-strength liquid medium; rooting and rhizome formation occurred when 0.5-1.0 mg IBA/litre was added. Coumarin, choline chloride, phloroglucinol and activated charcoal also induced rooting, either alone or with an auxin (IBA, NAA or IAA). Plantlets were hardened and established in soil.