Micropropagation of Dendrocalamus hamiltonii Munro using single node cuttings taken from elite seedling plants.
Single-node cuttings of precocious branchlets were cultured on MS medium as a source of explants (3-4 mm segments from newly initiated buds) for further culture. Direct shoot bud regeneration leading to multiple shoot initiation and/or callus formation was achieved on MS medium containing 1 mg/litre of each of benzyladenine and 2,4-D. Addition of 0.5 mg gibberellic acid (GA3)/litre to the medium encouraged shoot differentiation in callus cultures. Some albino and variegated shoots were obtained, although most were green. Rapid shoot multiplication was achieved in half-strength liquid medium; rooting and rhizome formation occurred when 0.5-1.0 mg IBA/litre was added. Coumarin, choline chloride, phloroglucinol and activated charcoal also induced rooting, either alone or with an auxin (IBA, NAA or IAA). Plantlets were hardened and established in soil.