Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

A detection method for Pseudomonas solanacearum in symptomless potato tubers and some data on its sensitivity and specificity.

Abstract

This method for detection of latent infections of P. solanacearum race 3 in potato is based on extraction of 200 heel ends of potato, followed by screening with an indirect antibody stain (IFAS) and (in IFAS-positive cases) a pathogenicity test on tomato (PT). In some sensitivity and specificity tests with >600 samples, its detection level was 104 cells/ml in IFAS and 102-104 cells/ml in PT; percentage recovery was 0.1-10% (10% at lower contamination levels); false-positive samples due to cross-reacting bacteria in IFAS were limited to only 2-3%, using 2 polyclonal antisera against whole cells of P. solanacearum. The advantages of this method in comparison with ELISA and selective media are discussed.