Maize chlorotic mottle virus in Hawaiian-grown maize: vector relations, host range and associated viruses.
Studies were conducted in Hawaii to identify potential above-ground arthropod vectors of maize chlorotic mottle virus (MCMV), to search for plants serving as reservoirs for MCMV, and also to search for associated viruses in maize that might cause corn lethal necrosis disease; MCMV transmission studies used 6 arthropod species found in MCMV-infected maize fields (Peregrinus maidis, Sardia pluto, Empoasca solana, Adoretus sinicus, Tetranychus sp. and Frankliniella williamsi). After a 1-3-day acquisition feed period on MCMV-infected maize plants, 5 arthropod species gave positive test results for MCMV by enzyme-linked immunosorbent assay (ELISA), but only the thrips (F. williamsi) were able to transmit MCMV to healthy maize plants. This is the first evidence of MCMV transmission by thrips; MCMV was found in all maize genotypes tested and was detected by infectivity tests and ELISA in all parts of the maize plant and in mature seed with 13-30% moisture. Fifteen other species of plants tested by ELISA were negative for MCMV, except for 1 sample of the grass Trichachne insularis, and 1 sample of neem. However, only sap from maize plant parts tested positively for MCMV by ELISA was capable of infecting healthy maize plants. From authors' summary. KEYWORDS: TROPAG | Zea mays | Cereal Crops | Zea mays | plant viruses | maize chlorotic mottle virus | vectors | hosts | disease surveys | Hawai.