Development and chromosomal localization of genome-specific markers by polymerase chain reaction in Brassica.
The application of RAPD (random amplification of polymorphic DNA sequence) markers to the study of Brassica genetics is reported. Forty-seven arbitrary decamer oligonucleotides were used as primers to amplify genomic DNA by polymerase chain reaction. Some of the amplified products were genome specific and could be found in both diploid and derived amphidiploid species. Of a total of 65 such markers, 16 were A genome, 37 B genome and 12 C genome specific. Of the 37 B-genome-specific markers, 11 were mapped on four independent chromosomes of B. nigra with the aid of existing B. napus-nigra disomic alien addition lines.