Characterization of disomic addition lines Brassica napus-Brassica nigra by isozyme, fatty acid, and RFLP markers.
Six B. napus/B. nigra disomic addition lines were characterized by isoenzyme, fatty acid and RFLP markers. The markers were arranged in 6 synteny groups, representing 6 of the 8 chromosomes present in the B. nigra genome. Synteny group 1 displayed high levels of linoleic and linolenic acids in the seeds of the B. nigra parent. Synteny group 3 accumulated higher levels of eicosenoic [gadoleic] and erucic acids than B. nigra. Three of the lines transmitted the alien chromosome to 100% of the progeny. The rest had variable transmission rates but all were above 50%. Most of the lines produced disomic addition plants in their progeny, suggesting pollen transmission of the alien chromosome. In addition to the marked lines, 6 others remained unmarked. These could be grouped into 2 classes according to their alien chromosome transmission. It is likely that they represent the 2 other B. nigra chromosomes that remained uncharacterized by the markers. No diploid individuals carrying B. nigra genome-specific markers were detected in the progenies studied.