Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

Quarantine procedure No. 26. Pseudomonas solanacearum. Inspection and test methods.

Abstract

Immunofluorescence and pathogenicity tests are described for the detection of P. solanacearum in potato tubers. A scheme for screening potatoes is presented. Details of visual inspection, sampling and extraction of potato tubers, indirect immunofluorescence antibody stain (IFAS), pathogenicity test on tomato (in confirmation of a positive IFAS result), isolation from test plants, identification of pure culture and determination of biovar or race are given.