Monoclonal antibodies to the mycoplasma-like organism (MLO) responsible for grapevine flavescence dorée.
Three hybridomas producing monoclonal antibodies specific for the flavescence dorée MLO were obtained after fusion between spleen cells from a mouse immunized against flavescence dorée MLO and Sp2/O-Ag-14 mouse myeloma cells. These hybridomas were selected in an indirect sandwich ELISA in which antibodies from rabbit and mouse were used. The 3 monoclonal antibodies were examined for reactivity towards the MLO causal agents of 15 plant yellows. None were implicated in a serological relationship between the MLO of these plant yellows and flavescence dorée MLO. Reactivity of the 3 monoclonal antibodies were checked towards 2 other isolates of flavescence dorée. One of them detected the wild isolates.