Mechanical transmission of Pittosporum vein clearing virus.
The virus, associated with vein clearing of P. tobira in several Mediterranean countries, was successfully transmitted by sap inoculation. Except for Chenopodium amaranticolor and Gomphrena globosa, only Solanaceous plants were susceptible. In expressed sap of Nicotiana spp., PVCV lost infectivity when diluted to 10-6, after heating for 10 min at 50-55 deg C and after 2 h storage at room temp. It was not aphid-transmitted. In thin-sectioned cells accumulations of PVCV particles were seen in perinuclear positions or in the ground cytoplasm within membrane-bounded enclaves. PVCV is a typical rhabdovirus with nuclear maturation.