Studies on Melilotus mosaic virus.
The virus found on M. indica during a survey around Aligarh, India, infected 18 plant spp. of 7 families on inoculation. Chenopodium amaranticolor and Tetragonia expansa were local lesion hosts. Thermal inactivation was at 60 deg C, dilution end point 10-3-10-4 and infectivity in vitro 96 h. The best medium for maintaining infectivity was 0.1 M phosphate buffer pH 7. The chloroform-phosphate procedure, using 0.5% thioglycolic acid, was the best method for purification. UV spectrum was typical of nucleoprotein and consisted of 1 centrifugal component with sedimentation of 148 S. The flexuous filamentous particles were c. 600-200 nm X 12 nm. Antiserum was obtained with a microprecipitin test titre of 512. The virus was serologically related to tobacco etch virus.