Purification and properties of Elderberry latent virus, one of four sap-transmissible viruses obtained from American Elder (Sambucus canadensis L.).
The 4 viruses transmitted from S. canadensis [51, 50, 2295] included cherry leaf roll, tomato black ring, elderberry virus A (with filamentous particles 650X15 nm) and elderberry latent virus (ELV), which produces no symptoms in elder and most other hosts. In Chenopodium quinoa sap ELV lost infectivity after dilution to 10-5-10-6, storage for 10 min at 85-90°C and 7 days at 18°. Infectivity of nucleic acid extract was destroyed by ribonuclease in 0.2 NaCl. Purified preparations, obtained from C. quinoa leaf extracts clarified with chloroform, precipitated at pH 5 and differentially centrifuged, contained numerous isometric particles c. 30 nm diam. and a few c. 17 nm. ELV sedimented in 0.0phosphate buffer as a major 112S component and a minor 48S one. There was no serological relationship with 27 other isometric plant viruses tested. The present cryptogram is R/1: */*: S/S: S/*.