The in vitro cultivation of tapeworms. I. Growth of Hymenolepisdiminuta (Cestoda: Cyclophyllidea).
Berntzen describes a method for the in vitro cultivation of Hymenolepis diminuta which involves the continuous flow of a liquid medium. Cysticercoids dissected from beetles and excysted in Tyrode's solution were cultured to adults with proglottides containing oncospheres. The | culture medium consists of whole human blood plasma and Tyrode's solution to which are added DL cysteine hydrochloride, DL thyroxine, thiamin chloride, nicotínic acid, methyl testosterone, riboflavin, a yeast extract and chick embryo extract in that order. Whereas there was little change in length of the worms between the third and fourth days of culture, a sharp daily increase in total length was seen between the fourth and fifteenth days. J.W.S.