Tissue culture processes have been established for Dendrobium wilsonii Rolfe, a valued ornamental and medicinal orchid. Pods aged 180 days served as seed sources. These seeds underwent surface sterilization and were then aseptically germinated on Murashige and Skoog (MS) medium supplemented with...

Gastrochilus japonicus (Makino) Schltr. is one of the endangered species in Japan. The aim of this study was to establish an ex-situ conservation method of this species through asymbiotic culture of seeds using media with defined substances without using plant growth regulators. We investigated the ...

Objective: To evaluate the economic and financial viability for the implementation of a biofactory responsible for the in vitro propagation of Vanilla planifolia with TIB® bioreactors. Design/methodology/approach: A completely randomized design was used. The data were processed with the IBM SPSS...

In vitro regeneration was studied to protect the rare Chinese medicinal orchid Liparis nervosa (Thunb.) Lindl. The mixtures of protocorm and seeding and the stem tip were used as explants. The results revealed that the best essential medium for L. nervosa growth was 1/3 MS medium with 25 g . L- ¹...

Orchids are universally popular due to the wide range of colors, sizes, shapes, and scents in their flowers. The demand for orchids as cut flowers and pot plants is increasing in the flower market. Seed germination of orchids is comparatively low in the wild as they typically require fungal...

The epiphytic orchid species known as Cymbidium mastersii can only be found in the region of India known as Northeast India. The preservation of wild populations faces a significant obstacle in the form of a significant threat when it comes to the necessity of obtaining an additional source of the...

The germination in vitro is a successful technique for non-endospermic seeds. In this study, a novel method was developed for the mass production of Dendrobium anosmum 'Di Linh' plantlets. Using MS medium supplemented with 0.2 mg l^-1 BA, 99% of the seeds germinated and formed protocorm in a net...

Trichopilia suavis is one of the important representatives of the genus Trichopilia, which can be used as a potted plant for the interior design. also, its beautiful inflorescences are added unusual element to bouquet compositions and actively used in the production of perfumes. This study was...

Direct and indirect shoot regeneration in Vanilla odorata and indirect for V. pompona are described for the first time. Both species produce fragrant, vanillin-containing seed pods and are closely related to the commercially cultivated V. planifolia. Stem nodal segments cultured on Murashige and...

D. stratiotes is one of the endemic dendrobiums from Indonesia that grows in lowland rainforests. D. stratiotes is listed as a threatened orchid species due to the lack of available data, which results in a high uncertainty and insufficient information. This study aims to provide preliminary...