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CABI Book Chapter

Biotechnology and plant disease management.

Book cover for Biotechnology and plant disease management.

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Chapter 9 (Page no: 195)

Molecular diagnostics of plant-parasitic nematodes.

Biochemical and molecular methods of identification provide accurate, reliable diagnostic approaches for the identification of plant-parasitic nematodes. Initially, the techniques were used solely for taxonomic purposes, but increasingly became popular as a component of diagnostic information for farmers, growers and advisors. Diagnostic procedures are now available to differentiate the plant-pathogenic species from related but non-pathogenic species. The microscopic size of plant-parasitic nematodes poses problems and techniques have been developed to enrich samples to obtain qualitative and quantitative information on individual species. In addition, techniques are available to evaluate single nematodes, cysts or eggs of individual species in extracts from soil and plant tissue. Background information on early, pioneering work is presented as a prelude to discussion of diagnostic approaches. These include the use of isoelectric focusing (IEF) and restriction fragment length polymorphisms (RFLPs), progressing to antibody approaches and current polymerase chain reaction (PCR)-based techniques. DNA- or RNA-based techniques are the most widely used approaches for identification, taxonomy and phylogenetic studies, although the development and use of other methods has been, and in some cases still is, important. DNA bar coding and the extraction of DNA from preserved specimens will aid considerably in diagnostic information and these are discussed in the context of the future requirements of accurate and rapid diagnostic protocols.

Other chapters from this book

Chapter: 1 (Page no: 1) Signal transduction pathways and disease resistant genes and their applications to fungal disease control. Author(s): Xing, T.
Chapter: 2 (Page no: 16) Modulating quorum sensing and type III secretion systems in bacterial plant pathogens for disease management. Author(s): Yang, C. H. Yang, S.
Chapter: 3 (Page no: 58) Application of biotechnology to understand pathogenesis in nematode plant pathogens. Author(s): Mitchum, M. G. Hussey, R. S. Davis, E. L. Baum, T. J.
Chapter: 4 (Page no: 87) Interactions between plant and virus proteomes in susceptible hosts: identification of new targets for antiviral strategies. Author(s): Sanfaçon, H. Jovel, J.
Chapter: 5 (Page no: 109) Mechanisms of plant virus evolution and identification of genetic bottlenecks: impact on disease management. Author(s): Roossinck, M. J. Ali, A.
Chapter: 6 (Page no: 125) Molecular understanding of viroid replication cycles and identification of targets for disease management. Author(s): Owens, R. A.
Chapter: 7 (Page no: 146) Molecular diagnostics of soilborne fungal pathogens. Author(s): Lévesque, C. A.
Chapter: 8 (Page no: 165) Molecular detection strategies for phytopathogenic bacteria. Author(s): Boer, S. H. de Elphinstone, J. G. Saddler, G. S.
Chapter: 10 (Page no: 227) Molecular diagnostic methods for plant viruses. Author(s): Olmos, A. Capote, N. Bertolini, E. Cambra, M.
Chapter: 11 (Page no: 250) Molecular identification and diversity of phytoplasmas. Author(s): Firrao, G. Conci, L. Locci, R.
Chapter: 12 (Page no: 277) Molecular detection of plant viroids. Author(s): Singh, R. P.
Chapter: 13 (Page no: 301) Application of cationic antimicrobial peptides for management of plant diseases. Author(s): Misra, S. Bhargava, A.
Chapter: 14 (Page no: 321) Molecular breeding approaches for enhanced resistance against fungal pathogens. Author(s): Knox, R. E. Clarke, F. R.
Chapter: 15 (Page no: 358) Protein-mediated resistance to plant viruses. Author(s): Uhrig, J. F.
Chapter: 16 (Page no: 374) Transgenic virus resistance using homology-dependent RNA silencing and the impact of mixed virus infections. Author(s): Ravelonandro, M.
Chapter: 17 (Page no: 395) Molecular characterization of endogenous plant virus resistance genes. Author(s): Lanfermeijer, F. C. Hille, J.
Chapter: 18 (Page no: 416) Potential for recombination and creation of new viruses in transgenic plants expressing viral genes: real or perceived risk? Author(s): Fuchs, M.
Chapter: 19 (Page no: 436) Virus-resistant transgenic papaya: commercial development and regulatory and environmental issues. Author(s): Suzuki, J. Y. Tripathi, S. Gonsalves, D.
Chapter: 20 (Page no: 462) Potential disease control strategies revealed by genome sequencing and functional genetics of plant pathogenic bacteria. Author(s): Charkowski, A. O.
Chapter: 21 (Page no: 498) Molecular assessment of soil microbial communities with potential for plant disease suppression. Author(s): Elsas, J. D. van Costa, R.
Chapter: 22 (Page no: 518) Enhancing biological control efficacy of yeasts to control fungal diseases through biotechnology. Author(s): Marchand, G. Clément-Mathieu, G. Neveu, B. Bélanger, R. R.
Chapter: 23 (Page no: 532) Molecular insights into plant virus-vector interactions. Author(s): Rochon, D.

Chapter details

  • Author Affiliation
  • Plant Pathogen Interaction Division, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK.
  • Year of Publication
  • 2007
  • ISBN
  • 9781845932886
  • Record Number
  • 20083096366