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CABI Book Chapter

Citrus genetics, breeding and biotechnology.

Book cover for Citrus genetics, breeding and biotechnology.


This book is intended to provide consolidated information on citrus breeding in the era of biotechnology, which is likely to hasten the pace of variety development aimed at resolving the problems faced by grove owners growing currently available cultivars. The subjects covered are focused on citrus while providing information equally useful to the breeders of other tree crops. It will also help st...


Chapter 15 (Page no: 329)

Genetic transformation.

This chapter discusses the development of efficient and reliable transformation systems for many economically important citrus species, including sweet orange, sour orange, lime, lemon, citrange, alemow and mandarin, based on the selection of appropriate Agrobacterium strain for super-transforming citrus, the establishment of the most appropriate infection and co-cultivation conditions and culture media, including adequate selection conditions and culture media, the use of source plant material in a good ontological state, the determination of the competent cells for transformation in citrus explants, the use of appropriate marker genes and the rapid production of whole transgenic plants through grafting of regenerating transgenic shoots on to vigorous rootstocks first in vitro and later in the greenhouse. Current research on incorporating transgenes of potential agricultural interest into citrus species to obtain resistance to Citrus tristeza virus, higher tolerance to Phytophthora, higher tolerance to salinity and shortening the juvenile period are also mentioned.

Other chapters from this book

Chapter: 1 (Page no: 1) Citrus breeding: introduction and objectives. Author(s): Khan, I. A. Kender, W. J.
Chapter: 2 (Page no: 9) A comprehensive citrus genetic improvement programme. Author(s): Gmitter, F. G., Jr. Grosser, J. W. Castle, W. S. Moore, G. A.
Chapter: 3 (Page no: 19) Origin and taxonomy. Author(s): Nicolosi, E.
Chapter: 4 (Page no: 45) Citrus germplasm resources. Author(s): Krueger, R. R. Navarro, L.
Chapter: 5 (Page no: 141) Nucellar embryony. Author(s): Kepiro, J. L. Roose, M. L.
Chapter: 6 (Page no: 151) Cytogenetics. Author(s): Jaskani, M. J. Omura, M. Khan, I. A.
Chapter: 7 (Page no: 167) Haploidy. Author(s): Germanà, M. A.
Chapter: 8 (Page no: 197) Seedlessness and ploidy manipulations. Author(s): Ollitrault, P. Froelicher, Y. Dambier, D. Luro, F. Yamamoto, M.
Chapter: 9 (Page no: 219) Somaclonal variation in sweet orange: practical applications for variety improvement and possible causes. Author(s): Grosser, J. W. Deng, X. X. Goodrich, R. M.
Chapter: 10 (Page no: 235) Somatic hybridization. Author(s): Ollitrault, P. Guo WenWu Grosser, J. W.
Chapter: 11 (Page no: 261) Microprotoplast-mediated chromosome transfer and its potential for citrus breeding. Author(s): Louzada, E. S.
Chapter: 12 (Page no: 275) Mapping and marker-assisted selection. Author(s): Roose, M. L.
Chapter: 13 (Page no: 287) Cloning and characterization of disease resistance genes. Author(s): Gmitter, F. G., Jr. Deng ZhaNao Chen ChunXian
Chapter: 14 (Page no: 307) Genetic transformation of citrus for pathogen resistance. Author(s): Febres, V. J. Lee, R. F. Moore, G. A.
Chapter: 16 (Page no: 345) Mutation breeding. Author(s): Roose, M. L. Williams, T. E.
Chapter: 17 (Page no: 353) Shoot-tip grafting in vitro: impact in the citrus industry and research applications. Author(s): Navarro, L. Juárez, J.