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CABI Book Chapter

Seed biology: advances and applications. Proceedings of the Sixth International Workshop on Seeds, Merida, Mexico, 1999.

Book cover for Seed biology: advances and applications. Proceedings of the Sixth International Workshop on Seeds, Merida, Mexico, 1999.



Chapter 22 (Page no: 231)

Gene expression prior to radicle emergence in imbibed tomato seeds.

In tomato (Lycopersicon esculentum) seeds, the primary control of germination resides in the tissues of the radicle tip and the endosperm cap enclosing it. It is a reasonable assumption that germination is regulated ultimately by specific gene expression in these tissues. Two approaches were utilized to identify genes specifically associated with the events initiated by seed imbibition and culminating in radicle emergence. The genes for proteins or enzymes that were expected to play a role in germination were cloned using PCR or library-screening methods, including genes for several cell wall hydrolases (endo-β-mannanase, cellulase, polygalacturonase, xyloglucan endotransglycosylase, β-1,3-glucanase, and chitinase), expansins and galactinol synthase. In addition, mRNA populations from both radicle tips and endosperm caps of the gibberellin-deficient (gib-1) tomato mutant were compared by differential cDNA display following imbibition in the presence or absence of gibberellin (GA). Additional mRNAs identified by this approach that are either expressed or repressed in these tissues in response to GA include ones coding for an arabinosidase, a vacuolar H+-translocating ATPase subunit, a GA-responsive transcript and the activating subunit of a protein kinase involved in sugar sensing. These studies are beginning to reveal the wide array of genes and biochemical processes that are involved in the transition from seed development to germination.

Other chapters from this book

Chapter: 1 (Page no: 3) Travels with seeds: a cartography of seed science. Author(s): Black, M.
Chapter: 2 (Page no: 11) Protein synthesis in seed germination: connecting external cell signals with the ribosome. Author(s): Jiménez, E. S. de
Chapter: 3 (Page no: 27) bZIP and DOF transcription factors in the regulation of gene expression in barley endosperm. Author(s): Carbonero, P. Vicente-Carbajosa, J. Mena, M. Oñate, L. Lara, P. Diaz, I.
Chapter: 4 (Page no: 43) Impact of amphiphile partitioning on desiccation tolerance. Author(s): Hoekstra, F. A. Golovina, E. A.
Chapter: 5 (Page no: 57) Unravelling the responses of metabolism to dehydration points to a role for cytoplasmic viscosity in desiccation tolerance. Author(s): Leprince, O. Hoekstra, F. A. Harren, F. J. M.
Chapter: 6 (Page no: 67) Soluble sugars in maturing pea seeds of different lines in relation to desiccation tolerance. Author(s): Górecki, R. J. Lahuta, L. B. Jones, A. D. Hedley, C. L.
Chapter: 7 (Page no: 75) The role of stachyose synthase in the oligosaccharide metabolism of legume seeds. Author(s): Richter, A. Hoch, G. Puschenreiter, M. Mayer, U. Peterbauer, T.
Chapter: 8 (Page no: 85) Compartmentation of abscisic acid in developing muskmelon (Cucumis melo L.) seeds. Author(s): Welbaum, G. E. Pavel, E. W. Hill, D. R. Gunatilaka, M. K. Grayson, R. L.
Chapter: 9 (Page no: 101) Involvement of ABA and GAs in the regulation of dormancy in developing sorghum seeds. Author(s): Benech-Arnold, R. L. Enciso, S. Sánchez, R. A. Carrari, F. Perez-Flores, L. Iusem, N. Steinbach, H. Lijavetzky, D. Bottini, R.
Chapter: 10 (Page no: 113) Irrigation and seed quality development in rapid-cycling Brassica. Author(s): Ellis, R. H. Sinniah, U. R. John, P.
Chapter: 11 (Page no: 123) Molecular genetic analysis of Arabidopsis seed quality. Author(s): Groot, S. P. C. Geest, A. H. M. van der Tesnier, K. Alonso-Blanco, C. Bentsink, L. Donkers, H. Koornneef, M. Vreugdenhil, D. Bino, R. J.
Chapter: 12 (Page no: 133) Analysis of the cell cycle in sugarbeet seed during development, maturation and germination. Author(s): Śliwińska, E.
Chapter: 13 (Page no: 141) Possible roles of phosphoenolpyruvate carboxylase and pyruvate kinase in assimilate partitioning in developing maize embryos. Author(s): Rodríguez-Sotres, R. Lara-Nuñez, A. Rodríguez-Penagos, M.
Chapter: 14 (Page no: 151) Effects of seed ageing on the enzymic antioxidant system of maize cultivars. Author(s): Bernal-Lugo, I. Camacho, A. Carballo, A.
Chapter: 15 (Page no: 161) Towards the ex situ conservation of aquatic angiosperms: a review of seed storage behaviour. Author(s): Hay, F. Probert, R. Marro, J. Dawson, M.
Chapter: 16 (Page no: 179) Treatment of immature embryos of maize with water reduces their storability and the desiccation tolerance of the scutellum. Author(s): Bochicchio, A. Mariotti, B. Berjak, P. Pammenter, N. W.
Chapter: 17 (Page no: 189) Maillard reactions cause browning in bean seed coats during ageing: inhibition by aminoguanidine. Author(s): Taylor, A. G. Min, T. G. Paine, D. H.
Chapter: 18 (Page no: 197) Effects of desiccation on the subcellular matrix of the embryonic axes of Quercus robur. Author(s): Mycock, D. J. Berjak, P. Finch-Savage, W. E.
Chapter: 19 (Page no: 205) Loss of viability in rye embryos at different levels of hydration: senescence with apoptotic nucleosome cleavage or death with random DNA fragmentation. Author(s): Boubriak, I. Naumenko, V. Lyne, L. Osborne, D. J.
Chapter: 20 (Page no: 215) The effect of drying rate on recalcitrant seeds: 'lethal water contents', causes of damage, and quantification of recalcitrance. Author(s): Pammenter, N. W. Berjak, P. Walters, C.
Chapter: 21 (Page no: 223) Conservation of genetic resources naturally occurring as recalcitrant seeds. Author(s): Berjak, P. Mycock, D. J. Walker, M. Kioko, J. I. Pammenter, N. W. Wesley-Smith, J.
Chapter: 23 (Page no: 253) Characterization of germination-related genes in Avena fatua seeds. Author(s): Johnson, R.
Chapter: 24 (Page no: 261) Cell cycle control during maize germination. Author(s): Vázquez-Ramos, J. M.
Chapter: 25 (Page no: 271) Recent advances in ABA-regulated gene expression in cereal seeds: evidence for regulation by PKABA1 protein kinase. Author(s): Walker-Simmons, M. K.
Chapter: 26 (Page no: 277) Lettuce endosperm weakening: a role for endo-β-mannanase in seed germination at high temperature. Author(s): Cantliffe, D. J. Nascimento, W. M. Sung, Y. Huber, D. J.
Chapter: 27 (Page no: 287) Effect of free Mg2+ on the kinetics of the plasma membrane H+-ATPase from dry and hydrated maize embryos. Author(s): Sánchez-Nieto, S. Rodríguez-Sotres, R. Gómez-Puyou, M. T. de Carballo, A. Gavilanes-Ruíz, M.
Chapter: 28 (Page no: 297) The barley scutellar peptide transporter: relationship to germination and loss of seed viability. Author(s): Waterworth, W. M. West, C. E. Daws, M. I. Bray, C. M.
Chapter: 29 (Page no: 309) Molecular cloning and possible role of an asparaginyl endopeptidase (REP-2) from germinated rice seeds. Author(s): Kato, H. Minamikawa, T.
Chapter: 30 (Page no: 315) Metabolism of essential oils during inhibition of wheat seed germination. Author(s): Dudai, N. Larkov, O. Mayer, A. M. Poljakoff-Mayber, A. Putievsky, E. Lerner, H. R.
Chapter: 31 (Page no: 323) Genetic model for dormancy in wild oat. Author(s): Foley, M. E.
Chapter: 32 (Page no: 329) Characterization and expression of two protein kinase genes and an EIN3-like gene, which are regulated by ABA and GA3 in dormant Fagus sylvatica seeds. Author(s): Lorenzo, O. Rodríguez, D. Nicolás, C. Nicolás, G.
Chapter: 33 (Page no: 341) Effect of fusicoccin and gibberellic acid on germination and α-amylase expression in barley grains. Author(s): Meulen, R. M. van der Vredenbregt-Heistek, J. Caspers, M. P. M. Wang, M.
Chapter: 34 (Page no: 347) Smoke and germination of arable and rangeland weeds. Author(s): Adkins, S. W. Davidson, P. J. Matthew, L. Navie, S. C. Wills, D. A. Taylor, I. N. Bellairs, S. M.
Chapter: 35 (Page no: 363) Intermittent germination: causes and ecological implications. Author(s): Cavers, P. B. Qaderi, M. M. Manku, R. Downs, M. P.
Chapter: 36 (Page no: 375) Seed ecology at the northern limit of the tropical rain forest in America. Author(s): Vázquez-Yanes, C. Orozco-Segovia, A. Sánchez-Coronado, M. E. Rojas-Aréchiga, M. Batis, A. I.
Chapter: 37 (Page no: 389) Genotypic and phenotypic germination survival strategies of ecotypes and annual plant species in the Negev desert of Israel. Author(s): Gutterman, Y.
Chapter: 38 (Page no: 401) Hydrothermal time as a tool in comparative germination studies. Author(s): Allen, P. S. Meyer, S. E. Khan, M. A.
Chapter: 39 (Page no: 411) Emergent weedy foxtail (Setaria spp.) seed germinability behaviour. Author(s): Dekker, J.
Chapter: 40 (Page no: 427) Biotechnological applications of seed biology. Author(s): Murphy, D. J.
Chapter: 41 (Page no: 439) Manipulating starch quality in seeds: a genetic approach. Author(s): Wang, T. L. Bogracheva, T. Y. Hedley, C. L.
Chapter: 42 (Page no: 449) Identification of germination-specific protein markers and their use in seed priming technology. Author(s): Job, D. Capron, I. Job, C. Dacher, F. Corbineau, F. Côme, D.
Chapter: 43 (Page no: 461) A critical assessment of the role of oligosaccharides in intracellular glass stability. Author(s): Buitink, J. Hoekstra, F. A. Hemminga, M. A.
Chapter: 44 (Page no: 467) Improvement of tomato seed germination by osmopriming as related to energy metabolism. Author(s): Corbineau, F. Özbingöl, N. Vinel, D. Côme, D.
Chapter: 45 (Page no: 477) Bio-osmopriming tomato (Lycopersicon esculentum Mill.) seeds for improved seedling establishment. Author(s): Warren, J. E. Bennett, M. A.
Chapter: 46 (Page no: 489) Use of threshold germination models under variable field conditions. Author(s): Finch-Savage, W. E. Phelps, K. Peach, L. Steckel, J. R. A.