The present study evaluated the cryoprotectant efficacy of dimethylacetamide (DMA) and ethylene glycol in a one-step protocol to freeze boar sperm. The sperm-rich portion of the ejaculates from two boars were collected once a week, for 10 weeks. After collection, the ejaculates were diluted (1:1;...

This study aimed to determine a pharmacokinetic profile for a single dosage of cyclosporine A (CsA) clinically used for immunosuppression in cats. Blood-CsA-concentrations were measured before and 1, 2, 4, 6, 8, 12 and 24 h after oral administration of 7 mg/kg body weight (BW) CsA (Atopica® oral...

Cold exposure influences liver metabolism, thereby affecting energy homeostasis. However, the gene regulatory network of the liver after cold exposure remains poorly understood. In this study, we found that 24 h cold exposure (COLD, 6°C) increased plasma glucose (GLU) levels, while reducing plasma...

Various microRNAs (miRNAs) present in autologous blood products of canines have not been studied recently. We aimed to elucidate the existence of miRNAs in platelet-rich fibrin (PRF) and the stability of canine autologous blood products under various storage conditions. Total RNAs were isolated...

In this investigation, we examined the efficiency of PRP as a therapy for inactive ovary (IO) in rabbits. IO in rabbits was induced by administration of D-galactose (200 mg/kg) (dissolved in saline, 0.2mL/rabbits/day) for ten consecutive days. Rabbits divide into three groups. After preparing two...

Intake of long-chain n-3 polyunsaturated fatty acids (PUFA) benefits human and animal health. Our study aimed to analyze the long-chain n-3 PUFA content of two types of food and their effect on plasma and erythrocyte phospholipids of Belgian Shepherd dogs. A total of 10 dogs were fed commercial...

The concentration of N^τ-methylhistidine in plasma provides an index of skeletal muscle protein breakdown. This study aimed to establish a quantitative method for measuring the concentrations of N^τ-methylhistidine and its isomer N^π-methylhistidine in chicken plasma, using liquid...

A sensitive, specific and rapid liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method was developed and validated to quantify triamcinolone acetonide and hydrocortisone concentrations in camel plasma. Samples were prepared by solid-phase extraction using Oasis HLB cartridges....

The pharmacokinetics and pharmacodynamics of a betamethasone (formulation dipropionate and sodium phosphate) were evaluated in 4 healthy camels after a single intramuscular dose of 35 µg/kg body weight. A sensitive, validated LC-MS/MS method for the quantification of plasma betamethasone and...

Microdialysis is a catheter-based method suitable for dynamic sampling of unbound antibiotic concentrations. Intravenous antibiotic concentration sampling by microdialysis has several advantages and may be a superior alternative to standard plasma sampling. We aimed to compare concentrations...