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Successful cryopreservation requires cells to tolerate volume excursions experienced during permeating cryoprotectant equilibration and during cooling and warming. However, prior studies have demonstrated that mouse spermatozoa are extremely sensitive to osmotically induced volume changes. A series ...

Agca, Y.; Gilmore, J.; Byers, M.; Woods, E. J.; Liu, J.; Critser, J. K.
Society for the Study of Reproduction, Madison, USA
Biology of Reproduction, 2002, 67, 5, pp 1493-1501