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Abstract

The fertilizing capacity of pure, fresh avian semen may disappear in just half an hour, hindering its successful use in artificial insemination (AI) projects. Longer storage requires the use of infra-physiological temperatures and of semen diluents that help preserve the spermatozoa but that do not ...

Author(s)
Santiago-Moreno, J.; Castaño, C.; Toledano-Díaz, A.; Esteso, M. C.; López-Sebastián, A.; Villaverde-Morcillo, S.; Dávila, S. G.; Gil, M. G.; Blesbois, E.
Publisher
Oxford University Press, Oxford, UK
Citation
Poultry Science, 2017, 96, 11, pp 4068-4074
Abstract

With avian sperm cryopreservation protocols, the most widely used cryoprotectants (CPAs) are the glycerol (GLY; in gradual freezing: in-straw freezing method), and the dimethylacetamide (DMA; in pellets by plunging into liquid nitrogen: in-pellet rapid freezing method). Use of both methods results...

Author(s)
Abouelezz, F. M. K.; Sayed, M. A. M.; Santiago-Moreno, J.
Publisher
Elsevier B. V., Amsterdam, Netherlands
Citation
Animal Reproduction Science, 2017, 184, pp 228-234
Abstract

Glycerol is a least toxic and most effective cryoprotectant for cryopreservation of poultry semen, but due to its contraceptive properties removal of glycerol is usually needed prior to artificial insemination. Dimethylsulfoxide (DMSO), a small amphiphilic molecule used as penetrating...

Author(s)
Rakha, B. A.; Ansari, M. S.; Akhter, S.; Zafar, Z.; Naseer, A.; Hussain, I.; Blesbois, E.; Santiago-Moreno, J.
Publisher
Elsevier, New York, USA
Citation
Theriogenology, 2018, 122, pp 61-67
Abstract

Glycerol (GLY) and egg yolk (EY) are good cryoprotectants of avian and mammalian sperm, but in birds, they strongly inhibit the eventual fertilization of ova. Using the sperm penetration (SP-holes) assay and fertility trials, the present study investigates (1) the possible mechanism by which this...

Author(s)
Abouelezz, F. M. K.; Castaño, C.; Toledano-Díaz, A.; Esteso, M. C.; López-Sebastián, A.; Campo, J. L.; Santiago-Moreno, J.
Publisher
Elsevier, New York, USA
Citation
Theriogenology, 2015, 83, 9, pp 1541-1547

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