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Abstract

We aimed at the evaluating the occurrence of macrophages in rabbit semen and finding possible relationship between macrophage concentration and spermatozoa quality. The concentration of macrophages in semen samples from broiler rabbit males of lines M91 and P91 (n=30) without overt evidence of...

Author(s)
Kuželová, L.; Vašíček, J.; Rafay, J.; Chrenek, P.
Publisher
Elsevier, New York, USA
Citation
Theriogenology, 2017, 97, pp 148-153
Abstract

The goal of this study was to evaluate the occurrence of macrophages in rooster semen and to investigate their impact on the spermatozoa quality. Ross 308 breeder males (n=30) with no evidence of genital tract infections were used to determine the concentration of macrophages using fluorescently...

Author(s)
Kuzelova, L.; Vasicek, J.; Chrenek, P.
Publisher
Wiley-Blackwell, Berlin, Germany
Citation
Reproduction in Domestic Animals, 2015, 50, 4, pp 580-586
Abstract

The objective of this study was to assess the effect of different diluent type and storage time on the quality of rooster insemination doses in terms of the spermatozoa motility and apoptosis (in vitro) and spermatozoa fertility potential (in vivo). The motility parameters were significantly (P<0.001) higher in the spermatozoa diluted in saline solution than those diluted in avian diluent at each storage time (0.5, 1 and 2 hours). The proportion of apoptotic spermatozoa was significantly (P<0.05) higher in the semen samples diluted in the avian diluent in comparison to the semen samples diluted in the saline solution after 1 hour of storage. The proportion of dead spermatozoa was significantly (P<0.05) higher in avian diluent in comparison to the semen samples diluted in the saline solution after 2 hours of storage. The fertility of spermatozoa diluted in the avian diluent significantly (P<0.01) decreased 1 hour after the ejaculate collection. In conclusion, saline solution maintained better quality of rooster spermatozoa in terms of their motility values and the proportion of apoptotic and dead cells (...

Author(s)
Vasicek, J.; Kuzelova, L.; Kulikova, B.; Chrenek, P.
Publisher
Science Reviews 2000 Ltd, St Albans, UK
Citation
Avian Biology Research, 2015, 8, 1, pp 41-46