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Abstract

With avian sperm cryopreservation protocols, the most widely used cryoprotectants (CPAs) are the glycerol (GLY; in gradual freezing: in-straw freezing method), and the dimethylacetamide (DMA; in pellets by plunging into liquid nitrogen: in-pellet rapid freezing method). Use of both methods results...

Author(s)
Abouelezz, F. M. K.; Sayed, M. A. M.; Santiago-Moreno, J.
Publisher
Elsevier B. V., Amsterdam, Netherlands
Citation
Animal Reproduction Science, 2017, 184, pp 228-234
Abstract

Glycerol (GLY) and egg yolk (EY) are good cryoprotectants of avian and mammalian sperm, but in birds, they strongly inhibit the eventual fertilization of ova. Using the sperm penetration (SP-holes) assay and fertility trials, the present study investigates (1) the possible mechanism by which this...

Author(s)
Abouelezz, F. M. K.; Castaño, C.; Toledano-Díaz, A.; Esteso, M. C.; López-Sebastián, A.; Campo, J. L.; Santiago-Moreno, J.
Publisher
Elsevier, New York, USA
Citation
Theriogenology, 2015, 83, 9, pp 1541-1547