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A method of freezing semen using N-methylacetamide (MA) as a cryoprotective agent was applied to Yakido, a chicken breed designated as a "Natural Monument" under the "Law for the Protection of Cultural Property" (Law No. 214, May 30, 1950) in Japan. Semen was collected from Yakido roosters, and...

Sasaki, K.; Tatsumi, T.; Tsutsui, M.; Niinomi, T.; Imai, T.; Naito, M.; Tajima, A.; Nishi, Y.
Japan Poultry Science Association, Ibaraki, Japan
Journal of Poultry Science, 2010, 47, 4, pp 297-301