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Twenty four ejaculates obtained from 4 native buffalos were used to study the effect of extender and thawing methods on sperm motility and acrosomal integrity during post thawing preservation for 4 h at 5 C. The extenders used were Tris Egg Yolk Citric Acid Fructose Glycerol (TEYCAFG), Illinois...
The comparative efficacy of the 4 extender (TEYCAFG, CUEEYG, IVTEEYG and MEEYG) was studied by split sample technique using 24 ejaculate (6 each from 4 buffalo bulls) after separation of seminal plasma. The percentage of motile sperm in frozen semen was significantly higher (p<0.01) in TEYCAFG...