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Abstract

Artificial insemination with cryopreserved spermatozoa is a major assisted reproductive technology in many species. In horses, as in humans, insemination with cryopreserved sperm is associated with lower pregnancy rates than those for fresh sperm, however, direct effects of sperm cryopreservation...

Author(s)
Ortiz-Rodriguez, J. M.; Ortega-Ferrusola, C.; Gil, M. C.; Martín-Cano, F. E.; Gaitskell-Phillips, G.; Rodríguez-Martínez, H.; Hinrichs, K.; Álvarez-Barrientos, A.; Román, Á.; Peña, F. J.
Publisher
Public Library of Sciences (PLoS), San Francisco, USA
Citation
PLoS ONE, 2019, 14, 6, pp e0213420
Abstract

Sperm quality was assessed in normozoospermic human (n=10) and Spanish breed stallion (n=10) after sperm fractionation during ejaculation. The first ejaculated fraction was separated from the second. A third sample was reconstituted using equivalent proportion of both fractions (RAW). Fraction 1,...

Author(s)
Torre, J. de la; Sánchez-Martín, P.; Gosálvez, J.; Crespo, F.
Publisher
Wiley, Berlin, Germany
Citation
Andrologia, 2017, 49, 8, pp e12708
Abstract

Background: Spermatozoa have a remarkable epigenome in line with their degree of specialization, their unique nature and different requirements for successful fertilization. Accordingly, perturbations in the establishment of DNA methylation patterns during male germ cell differentiation have been...

Author(s)
Perrier, J. P.; Sellem, E.; Prézelin, A.; Gasselin, M.; Jouneau, L.; Piumi, F.; Al-Adhami, H.; Weber, M.; Fritz, S.; Boichard, D.; Danvic, C. le; Schibler, L.; Jammes, H.; Kiefer, H.
Publisher
BioMed Central Ltd, London, UK
Citation
BMC Genomics, 2018, 19, 404, pp (29 May 2018)
Abstract

Many attempts have been made to identify laboratory tests that are predictive of sperm fertility, both to improve the quality of stallion semen doses for artificial insemination (AI) and to identify potential breeding sires if no fertility data are available. Sperm quality at the stud is mostly...

Author(s)
Morrell, J. M.; Stuhtmann, G.; Meurling, S.; Lundgren, A.; Winblad, C.; Garcia, B. M.; Johannisson, A.
Publisher
Elsevier, New York, USA
Citation
Theriogenology, 2014, 81, 8, pp 1005-1011
Abstract

Stallions are unique among livestock in that, like men, they commonly receive medical treatment for subfertility. In both species, about 15% of individuals have normal semen parameters but are subfertile, indicating a need for novel analyses of spermatozoa function. One procedure for improving...

Author(s)
Ing, N. H.; Forrest, D. W.; Love, C. C.; Varner, D. D.
Publisher
Elsevier, New York, USA
Citation
Theriogenology, 2014, 82, 2, pp 347-353
Abstract

Hoechst 33342 is the fluorophore used routinely to measure DNA in X- and Y-chromosome-bearing mammalian sperm so they can be separated by flow sorting. A difference of <3% in DNA mass can be detected. This synthetic dye consists of two adjacent benzimidazole rings with N-methyl-piperazine and phenolic groups at the ends. The molecule permeates the cell membrane of living cells and binds selectively to A-T base pairs exposed in the minor-groove of double stranded DNA. Capability to distinguish and separate X- and Y-chromosome-bearing sperm has led to artificial insemination of somewhere around a million female mammals. Offspring with obvious abnormalities are no more frequent...

Author(s)
Garner, D. L.
Publisher
Elsevier, New York, USA
Citation
Theriogenology, 2009, 71, 1, pp 11-21
Abstract

Our knowledge on the many aspects of mammalian reproduction in general and equine reproduction in particular has greatly increased during the last 15 years. Advances in the understanding of the physiology, cell biology, and biochemistry of reproduction have facilitated genetic analyses of...

Author(s)
Leeb, T.; Sieme, H.; Töpfer-Petersen, E.
Publisher
Elsevier, Amsterdam, Netherlands
Citation
Animal Reproduction Science, 2005, 89, 1/4, pp 21-29
Abstract

This attractively produced monumental work of reference is far above the competence of one writer and amply reflects the conjoint skills of its 21 contributors, of whom 15 are American. The book is addressed to " students of reproduction in agriculture, veterinary medicine, zoology and the...

Author(s)
HAFEZ, E. S. E.
Publisher
Philadelphia: Lea & Febiger. London : Baillière, Tindall & Cassell Ltd,
Citation
Reproduction and breeding techniques for laboratory animals., 1970, pp ix+ 375 pp.
Abstract

During 1959, 227 bulls, 18 boars, 6 stallions, 231 cows and heifers, 65 mares and 22 sows were examined for fertility. The main causes of infertility are indicated. White heifer disease was diagnosed in 7 out of 43 ♂ offspring (aged 12-22 mths. ) of 1 almost completely white bull whose dam belonged ...

Citation
Vlaams Diergeneeskundig Tijdschrift, 1960, 29, pp 54-64

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