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Abstract

The aim of the present study was the establishment of an effective protocol for cryopreservation of psittacine semen. Therefore, pooled semen samples of 30 cockatiels (Nymphicus hollandicus) were diluted with modified Lake diluent (1:4), partitioned into four equal parts. Three portions were mixed...

Author(s)
Schneider, H.; Fischer, D.; Failing, K.; Ehling, C.; Meinecke-Tillmann, S.; Wehrend, A.; Lierz, M.
Publisher
Elsevier, New York, USA
Citation
Theriogenology, 2018, 110, pp 8-17
Abstract

With avian sperm cryopreservation protocols, the most widely used cryoprotectants (CPAs) are the glycerol (GLY; in gradual freezing: in-straw freezing method), and the dimethylacetamide (DMA; in pellets by plunging into liquid nitrogen: in-pellet rapid freezing method). Use of both methods results...

Author(s)
Abouelezz, F. M. K.; Sayed, M. A. M.; Santiago-Moreno, J.
Publisher
Elsevier B. V., Amsterdam, Netherlands
Citation
Animal Reproduction Science, 2017, 184, pp 228-234
Abstract

Glycerol is a least toxic and most effective cryoprotectant for cryopreservation of poultry semen, but due to its contraceptive properties removal of glycerol is usually needed prior to artificial insemination. Dimethylsulfoxide (DMSO), a small amphiphilic molecule used as penetrating...

Author(s)
Rakha, B. A.; Ansari, M. S.; Akhter, S.; Zafar, Z.; Naseer, A.; Hussain, I.; Blesbois, E.; Santiago-Moreno, J.
Publisher
Elsevier, New York, USA
Citation
Theriogenology, 2018, 122, pp 61-67
Abstract

The population of red jungle fowl is declining and needs special attention for its conservation with suitable approaches. For ex situ in vitro conservation of Indian red jungle fowl, establishment of semen cryobank is an appropriate option, for which an extender with adequate retrieval capacity for ...

Author(s)
Rakha, B. A.; Ansari, M. S.; Akhter, S.; Hussain, I.; Blesbois, E.
Publisher
Elsevier B. V., Amsterdam, Netherlands
Citation
Animal Reproduction Science, 2016, 174, pp 45-55
Abstract

Glycerol (GLY) and egg yolk (EY) are good cryoprotectants of avian and mammalian sperm, but in birds, they strongly inhibit the eventual fertilization of ova. Using the sperm penetration (SP-holes) assay and fertility trials, the present study investigates (1) the possible mechanism by which this...

Author(s)
Abouelezz, F. M. K.; Castaño, C.; Toledano-Díaz, A.; Esteso, M. C.; López-Sebastián, A.; Campo, J. L.; Santiago-Moreno, J.
Publisher
Elsevier, New York, USA
Citation
Theriogenology, 2015, 83, 9, pp 1541-1547