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AbstractFull Text

The present study is focused on the methodology of fluorescence activated cell sorting (FACS) of spermatozoa stained by the antibody against extracellular surface marker ubiquitin (eUb) and subsequent protocol for their long term storage in liquid nitrogen (LN). High level of spermatozoa surface...

Author(s)
Petelák, A.; Krylov, V.
Publisher
Czech Academy of Agricultural Sciences, Prague, Czech Republic
Citation
Czech Journal of Animal Science, 2016, 61, 7, pp 310-316
Abstract

Frozen-thawed boar semen suffer a fertility decrease that negatively affects its widespread use. In recent years supplementing frozen-thawed boar sperm with different antioxidants gave interesting and promising results; the aim of the present work was to study the effect of supplementing boar sperm ...

Author(s)
Bucci, D.; Spinaci, M.; Yeste, M.; Mislei, B.; Gadani, B.; Prieto Martinez, N.; Love, C.; Mari, G.; Tamanini, C.; Galeati, G.
Publisher
Elsevier, New York, USA
Citation
Theriogenology, 2018, 117, pp 16-25
Abstract

We compared the effects of extenders of frozen-thawed semen on post-thaw sperm characteristics and the distribution of frozen-thawed spermatozoa in the female genital tract after fixed-timed deep intrauterine insemination (DIUI) in sows. Frozen semen samples were thawed and diluted in either...

Author(s)
Noguchi, M.; Yoshioka, K.; Hikono, H.; Suzuki, C.; Kikuchi, K.
Publisher
Elsevier B. V., Amsterdam, Netherlands
Citation
Animal Reproduction Science, 2015, 163, pp 164-171
Abstract

Eggs fertilised by spermatozoa marked by treatment with tris (1-aziridinyl) phosphine oxide (TEPA) show retarded cleavage and can be distinguished from normally developing eggs when recovered 5 days after ovulation. The fertilising ability of spermatozoa is not affected by marking with TEPA. In the ...

Author(s)
Pursel, V. G.; Johnson, L. A.
Citation
Proceedings, International Pig Veterinary Society, 4th International Congress, Ames, June 22-24, 1976., 1976, pp D18