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Abstract

Successful sex-sorting of goat spermatozoa and subsequent birth of pre-sexed kids have yet to be reported. As such, a series of experiments were conducted to develop protocols for sperm-sorting (using a modified flow cytometer, MoFlo SX®) and cryopreservation of goat spermatozoa. Saanen goat...

Author(s)
Bathgate, R.; MacE, N.; Heasman, K.; Evans, G.; Maxwell, W. M. C.; Graaf, S. P. de
Publisher
Wiley-Blackwell, Berlin, Germany
Citation
Reproduction in Domestic Animals, 2013, 48, 6, pp 893-898
Abstract

Flow cytometric sorting for the preselection of sex has progressed considerably in the 20 years since its inception. This technique has allowed the production of pre-sexed offspring in a multitude of species and become a commercial success in cattle around the world. However, due to the stress...

Author(s)
Graaf, S. P. de; Beilby, K. H.; Underwood, S. L.; Evans, G.; Maxwell, W. M. C.
Publisher
Elsevier, New York, USA
Citation
Theriogenology, 2009, 71, 1, pp 89-97
Abstract

Author(s)
Graaf, S. P. de; Evans, G.; Maxwell, W. M. C.; Cran, D. G.; O'Brien, J. K.
Publisher
Nottingham University Press, Nottingham, UK
Citation
Reproduction in domestic ruminants VI. Proceedings of the Seventh International Symposium on Reproduction in Domestic Ruminants, Wellington, New Zealand, 13-17 August, 2006, 2007, pp 473
Abstract

Author(s)
Graaf, S. P. de; Beilby, K. H.; Maxwell, W. M. C.; Evans, G.
Publisher
Nottingham University Press, Nottingham, UK
Citation
Reproduction in domestic ruminants VII. Proceedings of the Eighth International Symposium on Reproduction in Domestic Ruminants, Anchorage, Alaska, September 2010, 2010, pp 513
Abstract

Seminal plasma purportedly plays a critical role in reproduction, but epididymal spermatozoa are capable of fertilisation following deposition in the uterus, calling into question the biological requirement of this substance. Through a combination of direct observation of spermatozoa in utero using ...

Author(s)
Rickard, J. P.; Pini, T.; Soleilhavoup, C.; Cognie, J.; Bathgate, R.; Lynch, G. W.; Evans, G.; Maxwell, W. M. C.; Druart, X.; Graaf, S. P. de
Publisher
BioScientifica Ltd, Bristol, UK
Citation
Reproduction, 2014, 148, 5, pp 469-478
Abstract

Sex-sorting of mammalian spermatozoa has applications for genetic improvement of farm animals, in humans for the control of sex-linked disease, and in wildlife as a captive management strategy and for the re-population of endangered species. Considerable research has been undertaken worldwide on...

Author(s)
Maxwell, W. M. C.; Evans, G.; Hollinshead, F. K.; Bathgate, R.; Graaf, S. P. de; Eriksson, B. M.; Gillan, L.; Morton, K. M.; O'Brien, J. K.
Publisher
Elsevier Science B.V., Amsterdam, Netherlands
Citation
Animal Reproduction Science, 2004, 82/83, pp 79-95
Abstract

The fertility of ram spermatozoa cryopreserved prior to, and following, sex-sorting by flow cytometry was assessed after insemination of mature Merino ewes at a synchronised oestrus. Ewes were inseminated with spermatozoa from three rams, split into four treatment groups: 50×106 motile non-sorted,...

Author(s)
Graaf, S. P. de; Evans, G.; Maxwell, W. M. C.; Cran, D. G.; O'Brien, J. K.
Publisher
Elsevier, New York, USA
Citation
Theriogenology, 2007, 67, 2, pp 391-398
Abstract

The components of ruminant seminal plasma and their influence on the fertility of spermatozoa are reviewed. Seminal plasma can both inhibit and stimulate sperm function and fertility through the multifunctional actions of organic and inorganic components. These effects are now better understood...

Author(s)
Maxwell, W. M. C.; Graaf, S. P. de; Ghaoui, R. E.; Evans, G.
Publisher
Nottingham University Press, Nottingham, UK
Citation
Reproduction in domestic ruminants VI. Proceedings of the Seventh International Symposium on Reproduction in Domestic Ruminants, Wellington, New Zealand, 13-17 August, 2006, 2007, pp 13-38
Abstract

The fertility of ram spermatozoa that had undergone flow cytometric sorting (MoFlo® SX) and cryopreservation was assessed after low-dose insemination of synchronized Merino ewes. Oestrus was synchronized with progestagen-impregnated pessaries, PMSG and GnRH treatment. Ewes (n=360) were inseminated...

Author(s)
Graaf, S. P. de; Evans, G.; Maxwell, W. M. C.; Downing, J. A.; O'Brien, J. K.
Publisher
Blackwell Publishing, Berlin, Germany
Citation
Reproduction in Domestic Animals, 2007, 42, 6, pp 648-653
Abstract

Artificial insemination (AI) of sex-sorted sperm results in decreased fertility, compared with non-sorted sperm, in most species. However, this has not been the case in sheep, where the low-dose AI of sex-sorted ram sperm produced similar, if not superior, fertility to non-sorted controls. The aim...

Author(s)
Beilby, K. H.; Graaf, S. P. de; Evans, G.; Maxwell, W. M. C.; Wilkening, S.; Wrenzycki, C.; Grupen, C. G.
Publisher
Elsevier, New York, USA
Citation
Theriogenology, 2011, 76, 3, pp 471-481

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