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Various diluents and cooling rates were compared in trials involving the freezing of 600 rabbit ejaculates. A Tris diluent used in a 1:2 ratio proved superior to all other diluents tested. Semen should be cooled from room temp. to 5 deg C in not more than 1 h. Optimal thawing results were obtained...

Laiblin, C.; Rohloff, D.
Zuchthygiene, 1974, 9, 2, pp 89