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Boar semen was diluted 1:2 with an EDTA diluent, cooled to 15 deg C in 5 h, and centrifuged. The sediment was resuspended 1:2 in a lactose-yolk diluent and cooled to 5 deg in 2 h, when it was diluted 3:1 with a lactose-yolk-glycerol diluent and frozen in straws. After thawing for 50 s in water at...

Schrader, R.; Treu, H.; Hahn, R.
Zuchthygiene, 1976, 11, 2, pp 88