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Abstract

Following staining with fluorochrome DAPI (4,6-diamino-2- phenylindol), sperm left on the vitelline membrane of fertilized hen's eggs could be counted by fluorescence microscopy. This count was shown to be correlated with fertilization rates, and with the length of the fertile period (i.e. length...

Author(s)
Rohloff, D.; Schweighöfer, A.; Horst, P.
Citation
Berliner und Münchener Tierärztliche Wochenschrift, 1990, 103, 2, pp 37-39
Abstract

Author(s)
Laiblin, C.; Rohloff, D.
Citation
Tierarztliche Praxis, 1981, 9, 2, pp 237-244
Abstract

Author(s)
Rohloff, D.; Fischer, A.; Fischer-Pereira Cunha, A.
Citation
Berliner und Munchener Tieraztliche Wochenschrift, 1980, 93, 10, pp 189-191
Abstract

The sperm-rich fractions of 163 ejaculates collected from 6 Beagles, aged 2 yr, were frozen. Glycerol was a much better cryoprotectant than DMSO. It was better to add the glycerol at 5 deg C rather than at 25 deg . However, a combination of glycerol and DMSO gave best results. Forward motility...

Author(s)
Rohloff, D.; Laiblin, C.; Heidrich, S.
Citation
Berliner und Münchener Tierärztliche Wochenschrift, 1978, 91, 2, pp 31-33
Abstract

Author(s)
Rohloff, D.
Citation
Kleintier-Praxis, 1977, 22, 7, pp 289-292
Abstract

Author(s)
Rohloff, D.; Patel, B.
Citation
Zuchthygiene, 1976, 11, 2, pp 89
Abstract

478 ejaculates were collected from six 2-yr-old Beagle males , ejaculated twice weekly. Dilution with a Tris diluent proved superior to that with lactose and IVT diluents, and when glycerol was added before freezing, thawed ejaculates had a forward motility of 45-47%.

Author(s)
Rohloff, D.; Heidrich, S.
Citation
Zuchthygiene, 1976, 11, 2, pp 90
Abstract

Spermatozoa suitable for AI were obtained from the tail of the epididymis of killed Wistar rats and suspended in 0.2 ml Tris diluent. An average of 78.9 million spermatozoa was collected per male ; forward motility averaged 55.9% and percentage of live spermatozoa 44.1. The CR to intra-uterine ...

Author(s)
Rohloff, D.; Zeindl, E.; Bastiansen, L.
Citation
Berliner und Münchener Tierärztliche Wochenschrift, 1976, 89, 16, pp 318-321
Abstract

Various diluents and cooling rates were compared in trials involving the freezing of 600 rabbit ejaculates. A Tris diluent used in a 1:2 ratio proved superior to all other diluents tested. Semen should be cooled from room temp. to 5 deg C in not more than 1 h. Optimal thawing results were obtained...

Author(s)
Laiblin, C.; Rohloff, D.
Citation
Zuchthygiene, 1974, 9, 2, pp 89