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BACKGROUND: The cryopreservation process induces osmotic stress, membrane changes and production of reactive oxygen species resulting in damage to the spermatozoa. Together, the presence of oxygen in the extender aggravates the oxidative stress that further reduces the cryosurvival rate of sperm...
The purpose of this study was to examine the effects of sub-lethal concentration of Xanthine oxidase (XO) on the post-thawed bull sperm quality. Semen samples were collected from four Holstein bulls, twice a week and during three consecutive weeks (n=24 total ejaculates). After collection in each...
BACKGROUND: Semen cryopreservation might be influenced by sperm concentration. OBJECTIVES: This study was conducted to investigate the effect of three different semen concentrations [100 (C100), 50 (C50), and 25 (C25) × 106 spermatozoa/mL] on freezability of bull semen. METHODS: On each collection...
This work aimed to evaluate the effect of melatonin supplementation to a Tris-Gema extender on the kinetics and viability of cryopreserved bulls sperm. 54 ejaculates from Guzerá bulls were used. The sperm was diluted in TRIS-yolk supplemented with melatonin, at the following experimental...