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Addition of polyunsaturated fatty acids and/or cholesterol to a freezing diluent can modify the sperm plasma membrane composition, influencing its behavior during cryopreservation, thus, favoring seminal cryoresistance. The present study aimed to evaluate the effects of the addition of...
This work aimed to evaluate the effect of melatonin supplementation to a Tris-Gema extender on the kinetics and viability of cryopreserved bulls sperm. 54 ejaculates from Guzerá bulls were used. The sperm was diluted in TRIS-yolk supplemented with melatonin, at the following experimental...
This study was conducted to evaluate the effect of different concentrations of limonene (R)-(+) (50 µM, 100 µM and 150 µM) in supplementing the diluter of bulls freezing semen. Thirty-six ejaculated from four Curraleiro-Pé-Duro bulls were used for cryopreservation. The epifluorescence microscopy...
The purpose of this study was to examine the effects of sub-lethal concentration of Xanthine oxidase (XO) on the post-thawed bull sperm quality. Semen samples were collected from four Holstein bulls, twice a week and during three consecutive weeks (n=24 total ejaculates). After collection in each...