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Abstract

The long-term effects of post-ovulatory aging of mouse oocytes on reproductive fitness and longevity of offspring were analysed. Hybrid (C57BL/6JIco × CBA/JIco) parental generation (F0) females were artificially inseminated at 13 h (∼1 h post-ovulation) or 22 h (∼10 h post-ovulation) after GnRH...

Author(s)
Tarín, J. J.; Pérez-Albalá, S.; Pérez-Hoyos, S.; Cano, A.
Publisher
Society for the Study of Reproduction, Madison, USA
Citation
Biology of Reproduction, 2002, 66, 2, pp 495-499
Abstract

Mammalian diploid embryos are produced by fusion (fertilization) of specialized germ cells (gametes) from the male and female. The definitely differentiated male germ cells, the spermatozoa, gain the fertilizing ability after maturation in the epididymis. Therefore, under in vivo and conventional ...

Author(s)
Ogura, A.; Inoue, K.; Ogonuki, N.; Suzuki, O.; Mochida, K.; Matsuda, J.
Citation
Journal of Reproduction and Development, 1999, 45, Supplement, pp j37-j44
Abstract

Adult female mice were superovulated using PMSG and HCG or not superovulated and were mated to vasectomized males to induce pseudopregnancy. Caudal epididymal spermatozoa were aspirated from vasectomized males and activated with modified Earle's medium and 10% inactive rabbit serum. The...

Author(s)
Al-Dujaily, S. S.
Citation
Iraqi Journal of Veterinary Sciences, 1998, 11, 2, pp 257-264
Abstract

Background: Transgenic animals have become valuable tools for both research and applied purposes. The current method of gene transfer, microinjection, which is widely used in transgenic mouse production, has only had limited success in producing transgenic animals of larger or higher species. Here, ...

Author(s)
Chang KeeJong; Qian, J.; Jiang, M. S.; Liu, Y. H.; Wu MingChe; Chen ChiDar; Lai ChaoKuen; Lo HsinLung; Hsiao ChinTon; Brown, L.; Bolen, J., Jr.; Huang, H. I.; Ho PeiYu; Shih PingYao; Yao ChenWen; Lin WeyJinq; Chen ChungHsi; Wu FangYi; Lin YiJen; Xu, J.; Wang, K.
Publisher
BioMed Central Ltd, London, UK
Citation
BMC Biotechnology, 2002, 2, 5, pp (19 April 2002)
Abstract

Artificial insemination in the C3HeB/FeJ inbred strain of mice was shown to be more successful at the middle and end of the calendar year. This was found to be due to (1) an increase in the number of normal oestrous cycles exhibited by ♀♀ and (2) improved the phasing of ovarian and vaginal events....

Author(s)
Watson, J. G.; Chaykin, S.
Citation
Gamete Research, 1987, 16, 2, pp 171-176
Abstract

Oocytes at the metaphase II stage were collected from superovulated mice. In the first of 2 experiments, the oocytes were inseminated and transferred to medium containing KN-62 or KN-04 (an inactive form of KN-62) 70 min after insemination. KN-62, but not KN-04, suppressed second polar body...

Author(s)
Inagaki, N.; Suzuki, S.; Kitai, H.; Nakatogawa, N.; Kuji, N.; Iwahashi, K.; Yoshimura, Y.
Citation
Journal of Assisted Reproduction and Genetics, 1997, 14, 10, pp 609-616
Abstract

Males from which seminal vesicles and coagulating glands (SCC) had been removed and intact control ♂♂ were mated and oocytes were flushed from the ♀♀ 2 and 10 h after mating. The percentage of fertilized eggs at the 2 times was 0 and 3 respectively for treated ♂♂, and 21 and 98 for controls....

Author(s)
Suzuki, H.; Ueda, O.; Kamada, N.
Citation
Journal of Reproduction and Development, 1994, 40, 3, pp 177-182
Abstract

Pooled semen from mice was subjected to 1-step (12.5%) and 2-step (12.5 and 7.5%) bovine serum albumin (BSA) gradient separation. Adult female mice were inseminated with separated semen. Females inseminated with untreated spermatozoa served as controls. Sperm motility increased in 2-step BSA...

Author(s)
Archana Verma; Grewal, M. S.
Citation
International Journal of Animal Sciences, 1996, 11, 2, pp 423-424
Abstract

Mouse epididymal spermatozoa were frozen in 18% raffinose and 3% skim milk in distilled water and stored at -196°C, and later were thawed at room temp. Thawed spermatozoa in the solution were inseminated into the oviducts of pseudopregnant ♀♀ on the day when the vaginal plug first appeared. Five of ...

Author(s)
Nakagata, N.
Citation
Experimental Animals, 1992, 41, 4, pp 519-522
Abstract

Females of strain ICR were inseminated 1.5 h after ovulation with spermatozoa from DBA/2N, CF1 or C57BL/6N ♂♂, using sperm concentrations of 2 × 106 to 8× 106/µl. Eggs recovered from ♀♀ killed at intervals after insemination were examined for evidence of penetration. The intervals from insemination ...

Author(s)
Robl, J. M.; Dziuk, P. J.
Citation
Journal of Experimental Zoology, 1987, 242, 2, pp 181-187

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