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Abstract

MDA5 is the pivotal member of the retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) and is reported to play a crucial role in type I IFN-mediated responses against pathogen-associated molecular patterns (PAMPs), especially nucleic acids. In this study, we have identified and cloned the...

Author(s)
Paria, A.; Makesh, M.; Chaudhari, A.; Purushothaman, C. S.; Rajendran, K. V.
Publisher
Elsevier Ltd, Oxford, UK
Citation
Developmental and Comparative Immunology, 2018, 78, pp 71-82
Abstract

The platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF, PVF) family of proteins have been implicated in a wide range of biological functions in vertebrates, including cell proliferation, cell differentiation, cell migration, neural development and especially...

Author(s)
Huang XianDe; Zhang Hua; He MaoXian
Publisher
Elsevier Ltd, Oxford, UK
Citation
Gene, 2017, 637, pp 1-8
Abstract

The use of salbutamol (SAL), a β-adrenergic receptor agonist (β-agonist) that could enhance the lean meat-to-fat ratio in livestock, has been prohibited as an additive in animal feeds for livestock production in many countries due to its harmful effect to the consumers. Herein, we present a...

Author(s)
Yuan Yin; Zhao YingYing; Wu Kang; Yang Hong; Zhao Kang; Li JianGuo; Deng AnPing
Publisher
Royal Society of Chemistry, Cambridge, UK
Citation
Analytical Methods, 2017, 9, 39, pp 5806-5815
Abstract

Construction strategy and immune functions of recombinant herpesvirus of infectious bursal disease (IBD) were studied. Non essential region UL44-UL45 of the HVT FC126 genome was selected as insertion sites. Using the selection marker Eco-gpt (Xanthine-guanine phosphoribosyl transferase (gpt)),...

Author(s)
Pan QunXing; Bi ZhenWei; Wang YongShan; Ouyang Wei; Wang XiaoLi; Meng Kai; Xia XingXia; Zhu YuMei
Publisher
Chinese Veterinary Science, Lanzhou, China
Citation
Chinese Veterinary Science / Zhongguo Shouyi Kexue, 2017, 47, 9, pp 1073-1079
Abstract

In order to study the biological function of pig BST-2 gene, the BST-2 gene was amplified with specific primers from porcine kidney tissue, and molecular characterization of BST-2 nucleotide and amino acid sequence were analyzed with bioinformatics tools and online server. Then the prokaryotic...

Author(s)
Kong Ning; Wu YongGuang; Meng Qiong; Wang ZhongZe; Tong Wu; Zheng Hao; Li GuoXin; Shan TongLing; Zhou EnMin; Tong GuangZhi
Publisher
Editorial Board Chinese Journal of Veterinary Science, Changchun, China
Citation
Chinese Journal of Veterinary Science, 2017, 37, 8, pp 1594-1599, 1640
Abstract

In this study, the intestinal microbial flora diversity of adult and young African lions in the same breeding environment was detected by PCR-DGGE technique. Total bacterial DNA was extracted and 16S rDNA V3 region was amplified, then conducting PCR-DGGE. Subsequently, the specific bands of DGGE...

Author(s)
Jia XiaoYing; Wang Wei; Dong WenLong; Wang JiaZhen; Zhu ShiXin; Lou YuJie; Gao YunHang
Publisher
Editorial Board Chinese Journal of Veterinary Science, Changchun, China
Citation
Chinese Journal of Veterinary Science, 2017, 37, 8, pp 1507-1511
Abstract

VP1 gene encoded by the newly identified caprine enterovirus CEV-JL 14 was amplified and cloned to prokaryotic expression vector pGEX-4T-1. Recombinant GST-VP1 fusion protein was expressed, purified, emulsified with Freund's complete adjuvant and used to immunize the BALB/c mice following a...

Author(s)
Lu HaiBing; Wang MingYue; Zhu LiSai; Liu YaJing; Guo ChangMing; Zhang Qun; Yuan Yue; Tu ChangChun; Wang XinPing
Publisher
Editorial Board Chinese Journal of Veterinary Science, Changchun, China
Citation
Chinese Journal of Veterinary Science, 2017, 37, 8, pp 1468-1472
Abstract

To understand the genetic origin and variation of porcine epidemic diarrhea virus (PEDV) prevailing in Henan province, a total of 22 PEDV positive samples from suckling piglets with severe diarrhea were collected from 16 pig farms and amplified by RT-PCR. ORF3 genes were then cloned into pMD18-T...

Author(s)
Qiao Han; Zhao Li; Zhang Yu; Xu PengLi; Yang XingWu; Han HaoYing; Yang MingFan; Chen HongYing
Publisher
Editorial Board Chinese Journal of Veterinary Science, Changchun, China
Citation
Chinese Journal of Veterinary Science, 2017, 37, 8, pp 1451-1456, 1467
Abstract

In order to study genetic variation diversity of porcine circovirus type 2 (PCV2) strains in Shanxi, the genomic sequences of nine PCV2 strains including SXQX, SXCZ, SXTY2, SXJC, SXJX, SXLL, SXPY, SXPG and SXXY recently isolated from some areas of Shanxi from 2013 to 2016, was cloned, sequenced and ...

Author(s)
Wu Xin; Meng Fan; Yao JingMing; Fan ZhenHua; Wang JuanPing; Han YiChao; Mi RuiJuan; Xue YiPeng; Zhao Yue; Liu WenJun
Publisher
Editorial Board Chinese Journal of Veterinary Science, Changchun, China
Citation
Chinese Journal of Veterinary Science, 2017, 37, 8, pp 1442-1450
Abstract

Dirofilaria immitis is a mosquito-borne parasite that produces an inflammatory process in the wall of the blood vessels of its definitive host during cardiopulmonary dirofilariosis, known as proliferative endarteritis. Parasite antigens participate in the appearance of this inflammatory event,...

Author(s)
Diosdado, A.; Gómez, P. J.; Morchón, R.; Simón, F.; González-Miguel, J.
Publisher
Elsevier B.V., Amsterdam, Netherlands
Citation
Veterinary Parasitology, 2017, 247, pp 64-69

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