Pestiviruses, including classical swine fever virus, remain a concern for global animal health and are responsible for major economic losses of livestock worldwide. Despite high levels of vaccination, currently available commercial vaccines are limited by safety concerns, moderate efficacy, and...
Author(s)
Xu QianRu; Ma FanShu; Yang DaiChang; Li QingMei; Yan LiMing; Ou JiQuan; Zhang LongXian; Liu YunChao; Zhan Quan; Li Rui; Wei Qiang; Hu Hui; Wang YanAn; Li XueYang; Zhang ShenLi; Yang JiFei; Chai ShuJun; Du YongKun; Wang Li; Zhang ErQin; Zhang GaiPing
Publisher
Wiley, Oxford, UK
Citation
Plant Biotechnology Journal, 2023, 21, 12, pp 2546-2559
Tenvermectin (TVM) is a novel 16-membered macrolide compound isolated and purified from the fermentation broth of genetically engineered Streptomyces avermitilis strain MHJ1011. TVM and ivermectin were administered at the dose of 0.3 mg/kg body weight through a single subcutaneous injection route...
Author(s)
Li GuiYu; Cao XingYuan; Liao JianWei; Wei YanMing
Publisher
Wiley, Oxford, UK
Citation
Veterinary Medicine and Science, 2023, 9, 3, pp 1211-1216
Interspecies somatic cell nuclear transfer (iSCNT) has an immense potential to rescue endangered animals and extinct species like mammoths. In this study, we successfully established an Asian elephant's fibroblast cell lines from ear tissues, performed iSCNT with porcine oocytes and evaluated the ...
Author(s)
Tien Dat Nguyen; Li HongHui; Zhuang YiQuan; Chen BoWei; Kinoshita, K.; Jamal, M. A.; Xu KaiXiang; Guo JianXiong; Jiao DeLing; Tanabe, K.; Wei YunFang; Li Zhuo; Cheng WenMing; Qing YuBo; Zhao HongYe; Wei HongJiang
Publisher
Routledge, Philadelphia, USA
Citation
Animal Biotechnology, 2023, 34, 6, pp 1909-1918
Pseudorabies virus (PRV) is an important swine virus that has a significant impact on the global swine industry. PRV is a member of the herpesvirus family, specifically the alphaherpesvirus subfamily, and has been extensively utilized as a prototype herpesvirus. Notably, recent studies have...
Author(s)
Wang HaiMing; Qiao YangYang; Cai BingYan; Tan Ju; Na Lei; Wang Yu; Lu Hui; Tang YanDong
Publisher
Frontiers Media S.A., Lausanne, Switzerland
Citation
Frontiers in Veterinary Science, 2023, 10, July,
Porcine circovirus type 2 (PCV2) plays a key role in the etiology of PCV2-associated disease (PCVAD), and its predominant strain is PCV2d which is not completely controlled by most commercially available vaccines against PCV2a strains. Pseudorabies (PR) caused by pseudorabies virus (PRV) variants...
Author(s)
Xu, R. Q.; Wang, L. Q.; Zheng, H. H.; Tian, R. B.; Zheng, L. L.; Ma, S. J.; Chen, H. Y.
Publisher
Elsevier Ltd, Oxford, UK
Citation
Comparative Immunology, Microbiology & Infectious Diseases, 2023, 101,
Background: CRISPR-based toolkits have dramatically increased the ease of genome and epigenome editing. SpCas9 is the most widely used nuclease. However, the difficulty of delivering SpCas9 and inability to modulate its expression in vivo hinder its widespread adoption in large animals. Results:...
Author(s)
Jin Qin; Liu XiaoYi; Zhuang ZhenPeng; Huang JiaYuan; Gou ShiXue; Shi Hui; Zhao Yu; Ouyang Zhen; Liu ZhaoMing; Li Lei; Mao JunJie; Ge WeiKai; Chen FangBing; Yu ManYa; Guan YeZhi; Ye YingHua; Tang ChengCheng; Huang Ren; Wang KePin; Lai LiangXue
Publisher
BioMed Central Ltd, London, UK
Citation
Genome Biology, 2023, 24, 8, pp (17 January 2023)
Using CRISPR/Cas9 gene editing technology,a porcine kidney cell line with KLHL34 gene knocked out was constructed, and the effect of KLHL34 on foot-and-mouth disease virus(FMDV)replication was preliminarily explored,so as to provide experimental materials for studying the function of KLHL34 and the ...
Author(s)
Ran QianDong; Yang Fan; Zhang Wei; Cao WeiJun; Zheng HaiXue; Lin GuoZhen
Publisher
Chinese Veterinary Science, Lanzhou, China
Citation
Chinese Veterinary Science / Zhongguo Shouyi Kexue, 2023, 53, 7, pp 851-857
The transfection efficiency of clustered regularly interspaced short palindromic repeats (CRISPR)/Cas ribonucleoprotein complexes was compared using three nonviral vector transfection reagents: nonliposomal polymeric (TransIT-X2), lipid nanoparticle delivery (CRISPRMAX), and peptide (ProteoCarry)...
Author(s)
Lin QingYi; Takebayashi, K.; Torigoe, N.; Liu Bin; Namula, Z.; Hirata, M.; Tanihara, F.; Nagahara, M.; Otoi, T.
Publisher
Wiley, Tokyo, Japan
Citation
Animal Science Journal, 2023, 94, e13878,
The vascular endothelium of xenografted pig organs represents the initial site of rejection after exposure to recipient immune cells. In this study, we aimed to develop a promoter specific to porcine vascular endothelial cells as a step toward overcoming xenograft rejection. Transcriptome analysis...
Author(s)
Kim SangEun; Sun WuSheng; Oh Miae; Lee Seunghoon; No JinGu; Lee Haesun; Lee Poongyeon; Oh KeonBong
Publisher
MDPI Publishing, Basel, Switzerland
Citation
Genes, 2023, 14, 10,
Objective: The aim of this study was to investigate whether the immune adherence function of porcine erythrocyte complement receptor type 1-like (ECR1-like) could promote porcine alveolar macrophages (PAMs) to capture sensitized genetic engineering bacteria GFP-E. coli, in order to explain the...
Author(s)
Zhang Zheng; Ling XiaoYa; Fan KuoHai; Sun Na; Sun PanPan; Sun YaoGui; Li HongQuan; Yin Wei
Publisher
Scientia Agricultura Sinica, Beijing, China
Citation
Scientia Agricultura Sinica, 2023, 56, 19, pp 3905-3916