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Abstract

Rice-based peptide vaccine based on T cell epitopes acts as an ideal oral tolerogen for the treatment of type 1 allergic diseases. To improve production yields of oral tolerogen against Japanese cedar pollen allergy, hybrid peptide comprising seven predominant human T cell epitopes (7Crp) derived...

Author(s)
Entesari, M.; Wakasa, Y.; Zanjani, B. M.; Takaiwa, F.
Publisher
Springer, Dordrecht, Netherlands
Citation
Plant Cell, Tissue and Organ Culture, 2018, 133, 2, pp 275-287
Abstract

Along with the innovation of genetic transformation technology and the expansion of research scope, a great number of progress and new application fields had been made and reached by gene gun bombardment, a technique regarded as the backbone force of transgenic technology, and widely applied in...

Author(s)
Wang Jun; Fu AiGen; Xu Min; Wang YuHua
Publisher
Guangxi University, Guangxi, China
Citation
Genomics and Applied Biology, 2018, 37, 1, pp 459-468
Abstract

Cadmium (Cd) and arsenic (As) pollution in paddy soil and their accumulation in rice (Oryza sativa) pose serious threats to human health. Rice internally detoxifies these toxic metal and metalloid to some extent, resulting in their accumulation within the edible parts. However, the mechanisms of Cd ...

Author(s)
Yamazaki, S.; Ueda, Y.; Mukai, A.; Ochiai, K.; Matoh, T.
Publisher
Wiley, Oxford, UK
Citation
Plant Direct, 2018, 2, 1, pp 34
Abstract

Low cost vaccines against cysticercosis are needed to fight this parasitosis, especially in developing countries. Herein polycistron arrangements were designed to accomplish the simultaneous expression of multiple protective antigens from Taenia solium in the plant cell as an attractive biofactory...

Author(s)
Rosales-Mendoza, S.; Monreal-Escalante, E.; González-Ortega, O.; Hernández, M.; Fragoso, G.; Garate, T.; Sciutto, E.
Publisher
Elsevier B.V., Amsterdam, Netherlands
Citation
Journal of Biotechnology, 2018, 266, pp 124-132
Abstract

Many recombinant proteins have been produced successfully in a rice suspension-cultured expression system based on the αAmy3 promoter and signal peptide. Here, a transgenic rice cell line, H11, which can produce recombinant human serum albumin (rHSA) under conditions of sugar starvation, was...

Author(s)
Liu YuKuo; Lu ChiaWei; Chang JerYuan; Lu ChingFan; Tan ChiaChun; Huang LiFen
Publisher
Springer, Dordrecht, Netherlands
Citation
Plant Cell, Tissue and Organ Culture, 2018, 132, 2, pp 383-391
Abstract

Japanese cedar pollinosis is a seasonal allergic disease caused by two major pollen allergens: Cry j 1 and Cry j 2 antigens. To develop an oral vaccine to treat pollinosis, we constructed recombinant Lactococcus lactis harboring the gene encoding fused T cell epitopes from the Cry j 1 and Cry j 2...

Author(s)
Vinh Van Hoang; Ochi, T.; Kurata, K.; Arita, Y.; Ogasahara, Y.; Enomoto, K.
Publisher
Springer Berlin, Heidelberg, Germany
Citation
Applied Microbiology and Biotechnology, 2018, 102, 1, pp 261-268
AbstractFull Text

The use of genetically modified organisms (GMOs) as biofactories for the production of recombinant proteins of commercial interest is at present one of the major applications of 'molecular' biotechnology, and the business basis of many modern biotech companies. Since marketing in the early 1980s of ...

Author(s)
Vicente, O.; Boscaiu, M.
Publisher
University of Agricultural Sciences and Veterinary Medicine, Faculty of Biotechnology, Bucharest, Romania
Citation
Scientific Bulletin. Series F. Biotechnologies, 2017, 21, pp 308-313
Abstract

The immunogenicity of plant-made peroral vaccine against cervical cancer was studied in mice during 342 days after vaccination with the material of tomato fruits genetically transformed with HPV16 L1 Ω 5′UTR TMV. The immune response was found on day 4 after vaccination in blood serum of vaccinated...

Author(s)
Salyaev, R. K.; Rekoslavskaya, N. I.; Stolbikov, A. S.; Tretyakova, A. V.
Publisher
Pleiades Publishing, Moscow, Russia
Citation
Doklady Biochemistry and Biophysics, 2017, 474, 1, pp 186-188
Abstract

Sterol carrier protein 2 (SCP-2) plays important physiological roles in sterols absorption and transport in insects. In this study, the recombinant expression plasmid pET-22b/HaSCP2 was constructed and transformed into E. coli BL21 (DE3) for inducible expression. Induction conditions of the IPTG...

Author(s)
Du XinKai; Ren Juan; Hu Jun; Wang ChangGao; Lin JianGuo; Cai Jun; Du Xin
Publisher
Zhejiang Academy of Agricultural Sciences, Hangzhou, China
Citation
Acta Agriculturae Zhejiangensis, 2017, 29, 7, pp 1166-1171
Abstract

Agrobacterium-mediated transient expression systems enable plants to rapidly produce a wide range of recombinant proteins. To achieve economically feasible upstream production and downstream processing, it is beneficial to obtain high levels of two yield-related quantities of upstream production: ...

Author(s)
Fujiuchi, N.; Matsuda, R.; Matoba, N.; Fujiwara, K.
Publisher
Wiley, Hoboken, USA
Citation
Biotechnology and Bioengineering, 2017, 114, 8, pp 1762-1770

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