126 cutaneous mast cell tumours obtained by excisional biopsy from 106 dogs were evaluated for the presence of p53 protein using immunohistochemical staining. A standard avidin-biotin immunohistochemical protocol was used incorporating a polyclonal antibody of rabbit origin (CM-1) as the primary...

Pythiosis (caused by the aquatic oomycete Pythium insidiosum) is a devastating and often fatal cause of either severe transmural gastroenteritis or locally invasive subcutaneous disease in dogs living in the southeastern United States. Although early diagnosis is essential for successful treatment, ...

The purpose of this study was to evaluate the effects of sample handling, storage and culture techniques on the isolation of Pythium insidiosum from infected equine tissues. Tissue and kunker samples obtained immediately posteuthanasia from a horse with subcutaneous pythiosis from Louisiana, USA...

The molecular characteristics of the canine melanoma antigen recognized by IBF9 were determined by western blotting, enzymatic digestion studies and tunicamycin inhibition studies. Distribution of the antigen in 7 cultured melanoma cell lines was determined by flow cytometry. It was found that the...

Pond-reared fingerling red drum (Sciaenops ocellatus) showed greater than normal rates of mortality and poor physical condition. 52 fish were examined. Numerous 2 to 5 µm spherical organisms were lining the gastric microvillus border and clusters of 4 to 7 µm organisms were within epithelial cell...

Free, autogenous, full thickness skin grafts were applied to 10 dogs; 5 dogs were given an iron chelator, deferoxamine 10% hydroxyethyl pentafraction starch (DEF-HES; 50 mg/kg of body weight,i.v.), and 5 dogs were given an equal volume of 10% hydroxyethyl pentafraction starch (HES) in 0.9% saline...

A large primary retroperitoneal sublumbar neoplasm in a horse, with disseminated neoplastic foci in the brain, lung, kidney and spleen is described. The diagnosis was based on light microscopical studies and positive immunostaining for neuron-specific enolase. Because of the location of the primary ...

Free, autogenous, full-thickness skin grafting was performed on 10 dogs; 5 dogs were given an iron chelator; deferoxamine-10% hydroxyethyl pentafraction starch (DEF-HES; 50 mg/kg bwt i.v.) and 5 dogs were given 10% hydroxyethyl pentafraction starch (HES) in 0.9% saline solution (5 ml/kg, i.v.). The ...