Cookies on VetMed Resource

Like most websites we use cookies. This is to ensure that we give you the best experience possible.


Continuing to use  means you agree to our use of cookies. If you would like to, you can learn more about the cookies we use.

Sign up to receive our Veterinary & Animal Sciences e-newsletter, book alerts and offers direct to your inbox.

News Article

New test for Johne’s disease

Scientists develop quicker, more accurate test

A promising new bacteriophage-based test for Johne’s disease (JD) in dairy cattle has been developed at the Institute for Global Food Security (IGFS) and the School of Biological Sciences at Queen’s University Belfast.

The test promises to be both more rapid and sensitive in detecting Mycobacterium avium subsp. paratuberculosis (MAP) and is showing greater detection capability than the milk-ELISA test that is currently used. Crucially, it detects live infectious agent, not just antibodies against MAP as are detected by milk-ELISA.

In a recent study, the test was able to detect more infected animals by milk testing than milk-ELISA, so could potentially facilitate control of JD faster. As well as bovine milk, the new test can also be applied to faeces and blood from livestock.

The test was developed by Professor Irene Grant and her post-doctoral researcher Dr Antonio Foddai at IGFS and their research was published in Applied Microbiology and Biotechnology. They hope to now move to the applied stage of the science with further development and validation of their test for MAP infection at farm level.

Prof Grant said: “As farmers will know, JD is an endemic animal health issue worldwide, particularly in dairy herds. It is certainly present in Northern Ireland dairy herds but the true prevalence of JD in the local context is not accurately known.

“I hope our test will offer more accurate, rapid and quantitative results, and therefore help farmers and vets make more informed decisions about the infection status of animals in order to control the disease more effectively within herds.

"I also hope it will generate more accurate data on the prevalence of JD within Northern Ireland and therefore build a better picture of this animal-health problem.”

The test was developed as part of an Agri-Food Quest Competence Centre project, and was a collaboration between IGFS; AgriSearch; Biorex Food Diagnostics; and Dale Farm.

Article: Foddai, A. C. G., Grant, I. R. (2020). A novel one-day phage-based test for rapid detection and enumeration of viable Mycobacterium avium subsp. paratuberculosis in cows’ milk. Applied Microbiology and Biotechnology, 104, 9399-9412, doi: 10.1007/s00253-020-10909-0

Article details

  • Date
  • 02 December 2020
  • Source
  • Queen’s University Belfast
  • Subject(s)
  • Food Animals