Characterization of Cucumber mosaic virus infecting Indian long pepper (Piper longum L.) and betel vine (Piper betle L.) in India.
Cucumber mosaic virus (CMV) in Indian long pepper (P. longum) and betel vine (P. betle) was detected by reverse transcription polymerase chain reaction (RT-PCR). The coat protein gene sequences of CMV infecting these hosts were amplified. The resulting amplicons were cloned and sequenced. In both cases, the coat protein gene consisted of 657 nucleotides, potentially encoding for a protein of 218 amino acids. Sequence comparison revealed 100% identity of these isolates at the amino acid and nucleotide levels, suggesting a common origin. Sequence analyses with various CMV isolates showed that CMV infecting banana and black pepper in India were closer to isolates from Indian long pepper and betel vine. Based on coat protein gene sequence identities, CMV infecting Indian long pepper and betel vine belong to subgroup I. This is the first report of the molecular characterization of CMV infecting Indian long pepper and betel vine.