First report of a tospovirus in a commercial crop of Cape gooseberry in Brazil.
Eiras, M.; Costa, I. F. D.; Chaves, A. L. R.; Colariccio, A.; Harakava, R.; Tanaka, F. A. O.; Garcêz, R. M.; Silva, L. A.
Laboratory of Plant Virology, Instituto Biológico, 04014-002, São Paulo, SP, Brazil.
New Disease Reports 2012 Vol. 25 pp. 25
British Society for Plant Pathology, Reading, UK
Language of Text
A virus was isolated from a commercial crop of Cape gooseberry (Physalis peruviana) in October 2009 in Santa Maria, Rio Grande do Sul, Brazil. The disease was identified in a 5-ha cultivated area, where 100% of the plants were showing symptoms of stunting, mosaic, necrosis and foliar distortion. Spherical particles measuring 80-100 nm in diameter were observed by electron microscopy, giving the initial evidence that a tospovirus might be involved in the disease. Testing by plate trapped-ELISA, a positive reaction was only obtained from all five samples using anti-Tomato chlorotic spot virus (TCSV) antiserum. RT-PCR was performed using primers designed to conserved regions of the tospoviral S RNA. This generated 440 bp DNA fragments, whose sequence revealed a high degree of nucleotide identity with TCSV isolates from Argentina (98-99%) and Brazil (93-95%). Alignment of the partial coat protein nucleotide sequences showed that the Physalis TCSV isolate (GenBank accession no. JQ034525) clustered with other TCSV isolates, sharing a clade with Argentinean. This is thought to be the first report of the natural occurrence of TCSV in commercial crops of Cape gooseberry. To date, the disease is restricted to Rio Grande do Sul.