Occurrence of the Israel strain of Tomato yellow leaf curl virus in New Caledonia and Loyalty Islands.
Péréfarres, F.; Bruyn, A. de; Kraberger, S.; Hoareau, M.; Barjon, F.; Lefeuvre, P.; Pellegrin, F.; Caplong, P.; Varsani, A.; Lett, J. M.
CIRAD, UMR PVBMT, Pôle de Protection des Plantes, 97410 Saint-Pierre, Ile de La Réunion, France.
New Disease Reports 2012 Vol. 25 pp. 6
British Society for Plant Pathology, Reading, UK
Language of Text
In 2007, severe symptoms of leaf curling and yellowing resembling those of tomato yellow leaf curl disease were observed for the first time on tomato plants, with a high incidence in fields and greenhouses, in the south western region (Nouméa) of the Pacific island of New Caledonia. Tomato samples with leaf curling and yellowing symptoms were collected in the south west and west of New Caledonia, and also in Ouvéa (Loyalty Islands), respectively, in November and December 2010. Samples were tested for the presence of begomoviruses using a polymerase chain reaction (PCR) assay with a set of degenerate primers designed to amplify genomic regions of the Old World begomovirus DNA-A component. PCR products of the expected sizes were obtained, suggesting the presence of an Old World monopartite begomovirus. Full-length viral genomes were successfully amplified from six samples by rolling-circle amplification. The complete DNA-A genome sequences obtained (EMBL-GenBank-DDBJ accession nos. HE603241-HE603246) showed the highest pairwise sequence identity of 97.6-99.4% (BLAST, NCBI) with isolates of the Israel strain of Tomato yellow leaf curl virus (TYLCV-IL) from Spain and Reunion. This is thought to be the first report of the Old World TYLCV implicated in yellow leaf curl disease on tomato in New Caledonia and Loyalty Islands.