A new monopartite begomovirus species, Chilli leaf curl Vellanad virus, and associated betasatellites infecting chilli in the Vellanad region of Kerala, India.
Kumar, R. V.; Singh, A. K.; Chakraborty, S.
Molecular Virology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi - 110 0676, India.
New Disease Reports 2012 Vol. 25 pp. 20
British Society for Plant Pathology, Reading, UK
Language of Text
In December 2008, a chilli (Capsicum annuum) leaf curl disease incidence of up to 100% was recorded in Vellanad district of Kerala, India. The infected plants exhibited typical upward leaf curling, crinkling, puckering and reduction of leaf area, along with stunting of whole plants. Total DNA was isolated from chilli plants and applied as template for PCR amplification of begomovirus-specific fragments using degenerate primer pairs (PAL1v1978/PAR1c496) for the detection of Begomovirus DNA-A and Beta01/Beta02 for DNA-. Specific fragments of 1.5 kb for DNA-A and 1.4 kb for DNA-, respectively, were obtained from samples associated with symptoms. Following DNA amplification, the virus was considered a new begomovirus species for which the name Chilli leaf curl Vellanad virus (ChiLCVeV) is proposed. The associated betasatellites (GenBank accession nos. JN663876, 1355 nt and JN663877, 1372 nt) each contain a single ORF C1. They share maximum identity of 95.6 and 96.9% with Radish leaf curl betasatellite (EF175734) and Tomato leaf curl Bangladesh betasatellite (AY438558), respectively. The two molecules were identified as isolates of satDNAs. The results indicated that a new begomovirus species, ChiLCVeV, along with two distinct betasatellites (Radish leaf curl betasatellite and Tomato leaf curl Bangladesh betasatellite) are associated with chilli leaf curl disease in Vellanad, Kerala.