Abstract
Cotton and luffa plants with little leaf symptoms were observed during February 2010 at New Delhi, India. Nested polymerase chain reaction with phytoplasma 16S rDNA-specific primers efficiently detected the pathogen in the diseased plants. Sequence comparisons showed homology with the members of 'Candidatus Phytoplasma asteris', group 16SrI. Phylogenetic analysis also assigned the cotton and luffa little leaf phytoplasma to the 16SrI cluster.