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AbstractFull Text

Simple, rapid, high efficient and specific, thermal asymmetric interlaced PCR (TAIL-PCR), a kind of chromosome walking based on PCR, was mainly used to isolate the flanking sequences of target DNA segments. The principle, advantage, disadvantage and reaction system optimization of TAIL-PCR as well...

Author(s)
Ma Ling; Wang ZhiQiang; Qin ShaoMin; Wu JianMin
Publisher
Guangxi Academy of Agricultural Sciences, Nanning, China
Citation
Journal of Southern Agriculture, 2017, 48, 5, pp 926-932
AbstractFull Text

Objective: Rumen bacteria cellulase gene resources of Gayal were selected and enzymatic characteristics were studied from molecular biology level, so as to provide reference for development and utilization of new cellulases, and lay foundation for revealing the mechanism of cellulose degradation....

Author(s)
Yang TianLong; Wang ShuLing; Gu ZhaoBing; Zhu RenJun; Liu XuChuan; Zhang ChunYong; Yang ShuLi; Mao HuaMing; Leng Jing
Publisher
Guangxi Academy of Agricultural Sciences, Nanning, China
Citation
Journal of Southern Agriculture, 2017, 48, 5, pp 901-906
AbstractFull Text

Objective: To obtain the complete mRNA and promoter sequences of pig FAM213B gene, and provide a basis for studying the mechanism of the FAM213B gene in regulating gestation establishment and embryo development of female pigs. Method: The complete mRNA sequence of the FAM213B gene was obtained...

Author(s)
Zhang AiLing; Sun XianYue; Lu XiaoZhang; Li JiaQi; Zhang Hao
Publisher
South China Agricultural University, Guangzhou, China
Citation
Journal of South China Agricultural University, 2017, 38, 3, pp 1-8
Abstract

DNA methyltransferases (Dnmts) comprise a family of proteins which involved in the establishment and maintenance of DNA methylation patterns. In pig, the molecular characterization and tissue expression profile of Dnmt gene family are not clear. To solve this problem, reverse transcriptase PCR and...

Author(s)
Luo ZongGang; Zhang Kai; Chen Lei; Yang YuanXin; Fu PengHui; Wang KeTian; Wang Ling; Li MingZhou; Li XueWei; Zuo FuYuan; Wang JinYong
Publisher
Elsevier B.V., Amsterdam, Netherlands
Citation
Journal of Integrative Agriculture, 2017, 16, 6, pp 1367-1374
Abstract

As an alternative source of organs for transplantation into humans, attention has been directed to pigs due to their similarities in biological features and organ size. However, severe immune rejection has prevented successful xenotransplantation using pig organs and tissues. To overcome immune...

Author(s)
Kim, G. A.; Lee EunMi; Jin JunXue; Lee SangHoon; Taweechaipaisankul, A.; Hwang JongIk; Alam, Z.; Ahn, C.; Lee ByeongChun
Publisher
Springer, Dordrecht, Netherlands
Citation
Transgenic Research, 2017, 26, 4, pp 435-445
Abstract

Acinetobacter baumannii is recognised as a major pathogen of nosocomial infections that frequently show resistance to last-resort antimicrobials. To investigate whether A. baumannii from companion animals harbour carbapenem resistance mechanisms, 223 clinical isolates obtained from veterinary...

Author(s)
Ewers, C.; Klotz, P.; Leidner, U.; Stamm, I.; Prenger-Berninghoff, E.; Göttig, S.; Semmler, T.; Scheufen, S.
Publisher
Elsevier B. V., Amsterdam, Netherlands
Citation
International Journal of Antimicrobial Agents, 2017, 49, 1, pp 37-44
Abstract

Body size is a key determinant of fitness in Daphnia. Bigger size means higher feeding efficiency and reproduction. However, filamentous cyanobacteria have a more detrimental effect on larger daphnids. Predicted global warming is expected to reduce size of Daphnia and facilitate frequent occurrence ...

Author(s)
Dziuba, M. K.; Cerbin, S.; Wejnerowski, L.
Publisher
Springer, Dordrecht, Netherlands
Citation
Hydrobiologia, 2017, 798, pp 105-118
Abstract

Here we test for the possible coupling of two kairomone-induced, anti-fish defences in Daphnia, life-history changes (LHC) and diel vertical migration (DVM) mediated by the environmental factor light. A gradient of five different light intensities that represents naturally occurring intensities in...

Author(s)
Effertz, C.; Elert, E. von
Publisher
Springer, Dordrecht, Netherlands
Citation
Hydrobiologia, 2017, 798, pp 5-13
Abstract

This study was designed to explore the effects of histone deacetylase inhibitor romidepsin (FK228) treatment on the morphology, proliferation and karyotype of porcine foetal fibroblast cells and early developmental competence of somatic cell nuclear transfer (SCNT) embryos. We found that the...

Author(s)
Wang ZhangFan; Liu ChengXue; Hong RenYun; Yang Jie; Li Hui; Li YunSheng; Cao HongGuo; Zhang YunHai; Cao ZuBing
Publisher
Taylor & Francis, Abingdon, UK
Citation
Italian Journal of Animal Science, 2017, 16, 2, pp 189-198
Abstract

The purposes of this study were to analyze the bacterial diversity of dairy cattle rumen microflora and to screen for lipolytic clones of a metagenomic library constructed from the dairy cattle rumen contents using function-driven approach. The metagenomic DNA was extracted from dairy cattle rumen...

Author(s)
Liaw RenBao; Chen JoChing; Wu MingChe; Lee ChiaYin
Publisher
Taiwan Livestock Research Institute, Tainan, Taiwan
Citation
Journal of Taiwan Livestock Research, 2017, 50, 2, pp 124-133

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