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Abstract

Objective: The aim of the study was to clone the octamer-binding transcription factor 1, to investigate whether over-expression of Oct-1 regulated the major genes of coat color in melanocytes of mouse at the transcriptional levels and to explore its influence on the formation of melanin. Method:...

Author(s)
Yang YuJing; Nie RuiQiang; Xie JianShan; Fan RuiWen; Xu DongMei; Dong ChangSheng
Publisher
Scientia Agricultura Sinica, Beijing, China
Citation
Scientia Agricultura Sinica, 2017, 50, 4, pp 764-773
Abstract

(E)-β-Farnesene (EBF) is the common active component of aphid alarm pheromone. Either or both of two orthologs of ordorant-binding proteins (OBPs), OBP3 and OBP7, recently reported in aphids, may be involved in EBF perception. The aim of this study was to investigate the respondence of the aphid ...

Author(s)
Fan Jia; Xue WenXin; Duan HongXia; Jiang Xin; Zhang Yong; Yu WenJuan; Jiang ShanShan; Sun JingRun; Chen, J.
Publisher
Elsevier Ireland Ltd, Shannon, Irish Republic
Citation
Journal of Insect Physiology, 2017, 101, pp 151-160
Abstract

To screen potential protective antigen genes for developing genetic engineering vaccine of Gallibacterium anatis, a pair of primers were designed according to G. anatis UMN179 OmpA gene sequence published in Genbank, and the OmpA gene of G. anatis PDS-RZ-1-SLG was cloned and sequenced. In addition, ...

Author(s)
Wang Yan; Wang JiYang; Wang KunPeng; Peng ZhiFeng; Yang Xia; Wang ChuanQing
Publisher
Henan Agricultural Sciences, Zhengzhou, China
Citation
Journal of Henan Agricultural Sciences, 2017, 46, 8, pp 147-151
AbstractFull Text

Herein, we reported an Escherichia coli-based expression and purification method of recombinant porcine interferon alpha (rPoIFN-α). PoIFN-α coding sequence was cloned into pMD18-T vector and then subcloned into pET-32a (+) vector using standard recombinant DNA techniques and the resulting plasmid...

Author(s)
Zhao Jun; Yu HaiYang; Gan Lin; Zhao Yu; Li ShuQi; Fu XiuLe; Wang MingLi; Chen, J.
Publisher
Kafkas Üniversitesi, Veteriner Fakültesi Dergisi, Kars, Turkey
Citation
Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 2017, 23, 5, pp 825-829
Abstract

Prostaglandins (PGs) play a critical role in porcine reproduction, of which prostaglandin E2 (PGE2) and prostaglandin F2α (PGF2α) exert antiluteolytic and luteolysis actions, respectively. As a rate-limiting enzyme, carbonyl reductase 1 (CBR1) catalyzes the conversion of PGE2 to PGF2α. A high ratio ...

Author(s)
Zhang AiLing; Sun XianYue; Yin Qi; Zeng JianHua; Zhang Zhe; Li JiaQi; Zhang Hao
Publisher
Zhejiang University Press, Hangzhou, China
Citation
Journal of Zhejiang University (Science B), 2017, 18, 7, pp 626-634
Abstract

Molting is an important process for development and growth in arthropods. In crustaceans, molt is regulated by ecdysteroids or molting hormones that are synthesized in Y-organs. However, ecdysteroid biosynthesis pathway in crustaceans and its participating enzymes have not been well studied so far. ...

Author(s)
Sathapondecha, P.; Panyim, S.; Udomkit, A.
Publisher
Elsevier Ltd, Oxford, UK
Citation
Comparative Biochemistry and Physiology. A, Molecular & Integrative Physiology, 2017, 213, pp 11-19
Abstract

Constructed wetlands are important ecological tools for treating municipal, industrial, and agricultural wastewater. Terminal restriction fragment length polymorphisms and clone libraries were employed to determine the variation in bacterial community diversity and structure from a Myriophyllum...

Author(s)
Li Xi; Zhang MiaoMiao; Liu Feng; Li YuYuan; Li Yong; Xiao RuLin; Wu JinShui
Publisher
Elsevier B. V., Amsterdam, Netherlands
Citation
Applied Soil Ecology, 2017, 119, pp 56-63
Abstract

Spermiogenesis is a highly ordered and complex process in the male germ cell differentiation. The microtubule-based motor proteins KIF3A and KIF3B are required for the progression of the stages of spermiogenesis. In this study, the main goal was to determine whether KIF3A and KIF3B have a key role...

Author(s)
Zhao YongQiang; Yang HaiYan; Zhang DanDan; Han YingLi; Hou CongCong; Zhu JunQuan
Publisher
Elsevier B. V., Amsterdam, Netherlands
Citation
Animal Reproduction Science, 2017, 184, pp 59-77
Abstract

The gene encoding VP28 protein of WSSV was cloned into pRSET B vector, and recombinant VP28 (r-VP28) protein was produced in E. coli GJ1158 by induction with NaCl. The r-VP28 protein was purified. The purified r-VP28 protein was injected intramuscularly in Whiteleg shrimp, Litopenaeus vannamei, to...

Author(s)
Taju, G.; Madan, N.; Majeed, S. A.; Rajkumar, T.; Farook, M. A.; Vimal, S.; Basha, A. N.; Hameed, A. S. S.
Publisher
Springer, Dordrecht, Netherlands
Citation
Aquaculture International, 2017, 25, 5, pp 1761-1776
Abstract

Glanders is a disease of horses, donkeys and mules. The causative agent Burkholderia mallei, is a biorisk group 3 pathogen and is also a biothreat agent. Simple and rapid diagnostic tool is essential for control of glanders. Using a proteomic approach and immunoblotting with equine sera, we...

Author(s)
Dohre, S. K.; Aayushi Kamthan; Sandeep Singh; Alam, S. I.; Subodh Kumar
Publisher
Elsevier Ltd, Oxford, UK
Citation
Comparative Immunology, Microbiology & Infectious Diseases, 2017, 53, pp 26-32

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