In this study, prostatic acid phosphatase (PAP), which is overexpressed in human prostate cancer cells, was cloned to be fused to the IgM constant fragment (Fc) for enhancing immunogenicity and expressed in transgenic tobacco plants. Then, the transgenic plants were propagated by in vitro tissue...

Mussel adhesive protein (MAP), which is also named Mussel foot protein (Mfp), is secreted by Mytilidae's byssus gland. It is of high adhesiveness in water and is corrosion-resistant. At the same time, it is non-toxic, non-immunogenic and of excellent biocompatibility. Protein Mfp-5 has the...

The development of effective delivery systems to cope with the reduced immunogenicity of new subunit vaccines is a priority in vaccinology. Herein, experimental evidence supporting a papaya-based platform to produce needle-free, recombinant, highly immunogenic vaccines is shown. Papaya (Carica...

To research and develop rotavirus vaccine production in plant, the neutralization antigen gene VP7 was fused with CTB gene, and pPHAP1301-his-VP7-liner-CTB expression vecter was constructed and transformed into Arabidopsis thaliana. The his-VP7-liner-CTB was analyzed by RT-PCR, Western-blot and...

This chapter provides comprehensive information on genetic engineering studies that have introduced beneficial traits in tomato, such as improved fruit quality and enhancement of shelf life, abiotic stress tolerance, biotic stress tolerance and production of oral vaccines, among others.

Objective: The objective of this study was to heterologously express Arabidopsis thaliana magnesium transporter 10 (AtMGT10) in Escherichia coli and to generate polyclonal antibody against AtMGT10 in rabbits using recombinant AtMGT10 as the antigen. This work would lay foundation for the study of...

Achievements of cell and genetic engineering that led to formation of a new genetics chapter - transmission genetics - have been described. Results have been analyzed and new opportunities in the field of transgenomic somatic hybrids and cybrid obtaining, production of transgenic plants with...

An antifungal protein, AtUSP protein (At3 g53990), was isolated from Arabidopsis thaliana leaves by ion and size chromatography and sequenced by N-terminal sequencing. The AtUSP gene amplified from an Arabidopsis leaf cDNA library was transformed to Escherichia coli to express the AtUSP protein....

Lactoferrin and lysozyme are 2 glycoproteins with great antimicrobial activity, being part of the nonspecific defensive system of human milk, though their use in commercial products is difficult because human milk is a limited source. Therefore, many investigations have been carried out to produce...

Newcastle disease virus (NDV) is one of the most important viral pathogens of avian species and the causative agent of atypical fowl plague, a highly contagious and economically important disease characterized by high mortality rates and reduction of egg production. The HN and F proteins are the...